两种海蜇粘液糖蛋白的化学组成及抗氧化活性研究

发布时间：2018-03-01 17:04

本文关键词： 海蜇粘液糖蛋白化学组成抗氧化活性　出处：《大连工业大学》2015年硕士论文　论文类型：学位论文

【摘要】：海蜇粘液是海蜇加工生产过程中的废弃物,如能对其加以充分利用,将大大提高副产物的利用率,提高生产利润,并减少废弃物对环境的污染,形成一条更加完整的海蜇加工产业链。本实验主要对当地两种常见品种海蜇(营口绵蜇Rhopilema asamushi和大连沙蜇Stomopholus nomnrai)粘液的基本化学组成及抗氧化活性进行了初步的探究,以期海蜇粘液能够在食品、医药、化妆品等领域有所应用。将采集到的不同品种海蜇粘液样品经过滤、离心、透析、冻干等简单的预处理,得到了成分单一的糖蛋白物质,进一步对其化学组成进行了测定。选用冷冻干燥法测定水分含量;选用苯酚-硫酸法测定糖含量;蛋白质含量的测定采用考马斯亮蓝法;通过PMP柱前衍生化高效液相色谱法对其单糖构成进行检测;氨基酸组成情况通过液相色谱法检测;分子量选用SDS-PAGE电泳法测定;抗氧化活性以DPPH自由基清除率和羟自由基清除率为基础,借助ESR进行分析测定。经以上实验方法测定,营口绵蜇粘液的水分含量为94.85%,糖含量未经透析时为0.60%,经透析后为3.57%,蛋白含量未经透析时为2.61%,经透析后为33.54%;大连沙蜇粘液的水分含量为96.82%,总糖含量未经透析时为0.47%,经透析后为3.69%,蛋白含量未经透析时为1.52%,经透析后为31.25%。营口绵蜇粘液含氨基葡萄糖、甘露糖、木糖、氨基半乳糖、葡萄糖、核糖和半乳糖7种单糖;大连沙蜇粘液中检测到木糖、甘露糖、氨基半乳糖、氨基葡萄糖、葡萄糖和核糖6种单糖成分。营口绵蜇粘液中含有除蛋氨酸、半胱氨酸和色氨酸的15种氨基酸;大连沙蜇粘液中含有除蛋氨酸、半胱氨酸、色氨酸和组氨酸的14种氨基酸。两种海蜇粘液分子量相近,均约为97.2kDa。营口绵蜇粘液的DPPH自由基半清除率为17.51mg/m L,羟自由基半清除率为139.70mg/mL;大连沙蜇粘液的DPPH自由基半清除率为24.15mg/m L,羟自由基半清除率为150.19mg/m L。
[Abstract]:Jellyfish jellyfish is mucus production and processing process of waste, such as to make full use of them, will greatly improve the utilization rate of by-products, increase the production profit, and reduce waste pollution to the environment, the formation of a more complete industrial chain. The main processing jellyfish on the two common varieties of jellyfish (Yingkou cotton L Rhopilema asamushi and Dalian Stomopholus nomnrai nemopilema nomurai) composition and antioxidant activity of basic chemical mucus of the preliminary inquiry, in order to jellyfish mucus in food, medicine, cosmetics and other fields has been applied. The different varieties of jellyfish mucus samples collected by filtration, centrifugation, dialysis and freeze-drying simple pretreatment. The single component of the glycoprotein material, further on its chemical composition were determined. The determination of moisture content by freeze-drying method; Determination of sugar content by phenol sulfuric acid method; protein Determination of matter content by Coomassie brilliant blue method; by PMP pre column derivatization HPLC method to detect the composition of monosaccharide; detection of amino acid composition by liquid chromatography; molecular weight by SDS-PAGE electrophoresis; antioxidant activity DPPH radical scavenging and hydroxyl radical scavenging rate as the basis, with the help of ESR through the above analysis. The experimental method for the determination of moisture content of cotton, Yingkou sting mucus was 94.85%, sugar content without dialysis for 0.60%, after dialysis was 3.57%, protein content without dialysis for 2.61%, after dialysis was 33.54%; the moisture content of Dalian nemopilema nomurai mucus was 96.82%, the total sugar content is not after dialysis for 0.47%, after dialysis was 3.69%, protein content without dialysis for 1.52%, after dialysis for 31.25%. Yingkou cotton sting mucus containing amino glucose, mannose, xylose, galactosamine, glucose, ribose and galactose 7 Monosaccharide; xylose, detection of Dalian nemopilema nomurai in mucus, mannose, galactosamine, glucosamine, 6 kinds of monosaccharide containing glucose and ribose. Except for methionine in Yingkou cotton sting in mucus, 15 kinds of amino acid cysteine and tryptophan; Dalian nemopilema nomurai mucus containing except methionine, cysteine, 14 kinds of amino acids tryptophan and histidine. Two kinds of jellyfish mucus with similar molecular weight, were about DPPH radical 97.2kDa. Yingkou cotton sting mucus clearance rate was 17.51mg/m half L, hydroxyl radical scavenging rate of 139.70mg/mL - DPPH radical; Dalian nemopilema nomurai semi mucus clearance rate was 24.15mg/m L, the hydroxyl radical scavenging rate of half 150.19mg/m L.

【学位授予单位】：大连工业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：X792;TS201.21

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