体外生物测试工具在评价污水污泥毒性效应中的应用

发布时间：2018-03-20 09:52

本文选题：基线毒性　切入点：遗传毒性　出处：《环境工程学报》2016年12期 　论文类型：期刊论文

【摘要】：以上海地区7处典型污水处理厂产生的污水污泥为研究对象,利用体外生物测试工具,检测了污水污泥有机提取液的基线毒性、遗传毒性、雌激素效应和芳香烃受体效应水平,并结合化学分析对某些能引起毒性效应的有机污染物进行确认。结果表明,污水污泥有机提取液的基线毒性强度为133~3 401 mg·kg~(-1);遗传毒性强度从未检出到25 mg·kg~(-1);雌激素效应当量均低于0.02 ng·kg~(-1);芳香烃受体效应强度为73~6 838 ng·kg~(-1)。与国内外相关研究比较发现,基线毒性、遗传毒性、雌激素效应水平均较低,仅芳香烃受体效应水平接近于国内外污水污泥中芳香烃受体效应的水平。在对芳香烃受体效应水平的检测中发现:S1点检出的化学物质可以解释污水污泥70%的类二恶英效应,其中多环芳烃(PAHs)尤其是PAHs的单体Bk F占主导地位,而其余样点则不到10%的效应可以被解释,因此有待进一步对其余样点的化学污染物进行分析和确认。
[Abstract]:Taking sewage sludge from seven typical sewage treatment plants in Shanghai as the research object, the baseline toxicity, genetic toxicity, estrogen effect and aromatic hydrocarbon receptor effect of organic sewage sludge extracts were determined by in vitro biological test tools. Some organic pollutants which can cause toxic effects are confirmed by chemical analysis. The baseline toxicity of sewage sludge organic extract was 133n 3 401 mg 路kg -1; the genetic toxicity was not detected to 25 mg 路kg ~ (-1); the equivalent of estrogen effect was lower than 0.02 ng 路kg ~ (-1); the effect intensity of aromatic hydrocarbon receptor was 73 ~ 6 838 ng 路kg ~ (-1). The genotoxicity and estrogen effect were all low. Only the level of aromatics receptor effect was close to that of domestic and foreign sewage sludge. In the detection of the effect level of aromatic hydrocarbon receptor, it was found that the chemical substances detected at 1: S1 point could explain the dioxin-like effect of sewage sludge 70%. Among them, PAHs), especially the monomer BkF of PAHs, dominate, but the effect of less than 10% of the other samples can be explained. Therefore, the chemical pollutants of the other sites need to be further analyzed and confirmed.
【作者单位】：上海海洋大学水产动物遗传育种中心上海市协同创新中心;华东师范大学河口海岸学国家重点实验室;南京大学环境学院污染控制与资源化研究国家重点实验室;清华大学环境学院环境模拟与污染控制国家重点联合实验室;
【基金】：国家海洋局公益项目子课题(201105010-10)
【分类号】：X703;X171.5

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