聚吡咯覆膜改性电极对氢自养反硝化和产甲烷电极生物膜反应器的影响

发布时间：2018-04-01 11:05

本文选题：聚吡咯　切入点：电极改性　出处：《大连理工大学》2015年硕士论文

【摘要】：电极生物膜法作为将生物膜法和电化学法联合而发展起来的污水处理技术,近年来越来越受到研究者们的关注。其所具有的优势有：生物膜微生物固定生长且生物量较大；电化学电极过程拥有高效的氧化还原能力；电极与微生物之间具有较高传质速率。随着生物电化学反应器的发展,涉及到生物电化学反应器的电极改性被广泛研究。有研究报道,改性电极运用到生物电化学反应器中可以提高反应器性能。本论文探究利用电化学聚合方法在碳纤维毡电极上制备聚吡咯(polypyrole, PPy)的聚合效果,并利用傅里叶变换红外光谱(Fourier transform infrared, FTIR)及扫描电镜(SEM)对其进行表征；将改性后的碳纤维毡电极应用到电极生物膜反应器(Biofilm Electrode Reactor, BER)中,考察电极改性对电极生物膜反应器性能的影响并研究聚吡咯覆膜改性电极对生物膜附着量、胞外聚合物成分及生物膜微生物群落的影响。结果表明,以25 mM AQDS(蒽醌2,6-二磺酸钠)为掺杂剂的恒电压电化学聚合能够在碳纤维电极表面形成均匀稳定的聚吡咯膜,实现聚吡咯在碳纤维毡电极上的覆膜改性。改性后的电极应用到电极生物膜反应器中,可使自养反硝化反应器对N03--N的去除效率由对照反应器的67.3%增加到83.9%,处理效果提高了24.7%；可使产甲烷反应器COD去除率较其对照反应器最高增加约18%,甲烷产量较其增加了约3倍。对两种反应器内电极生物膜进行生物量测定和扫描电镜分析,可以看到应用聚吡咯覆膜改性电极的R2反应器中改性电极生物膜附着量均明显多于对照反应器R1中未改性电极生物膜的附着量,说明电极改性有利于生物膜的附着。生物膜胞外聚合物蛋白质和多糖的测定结果说明,电极改性有助于提高胞外聚合物的含量和蛋白质及多糖的比例,从而提高生物膜的活性及其强度和稳定性。氢自养反硝化电极生物膜微生物16S rDNA分析结果表明聚吡咯覆膜改性电极生物膜的优势菌为Hydrogenophaga sp(噬氢菌属)和Thauera sp(陶厄氏菌属),不同于对照电极生物膜菌落组成中的优势菌属Dechloromonas sp.,并且其生物膜的菌落组成更多样化。产甲烷电极生物膜微生物古菌16S rDNA分析结果表明两反应器电极生物膜菌落组成中优势菌属均为Methanosarcina sp(产甲烷八叠球菌),聚吡咯覆膜改性电极生物膜的优势菌属其次为Methanomicrobia sp(产甲烷微菌),而对照电极生物膜优势菌属其次为Methanobacterium sp(产甲烷杆菌),两反应器微生物菌属组成有明显差异。这说明电极材料的改性对电极生物膜微生物群落的组成产生了影响。
[Abstract]:Electrode biofilm, as a wastewater treatment technology which combines biofilm and electrochemical process, has attracted more and more attention in recent years.Its advantages are: biofilm microorganism growth and biomass is large; electrochemical electrode process has high redox ability; electrode and microorganism have a higher rate of mass transfer.With the development of bioelectric chemical reactor, electrode modification related to bioelectrochemical reactor has been widely studied.It has been reported that the modified electrode can improve the performance of the reactor.In this paper, the polymerization effect of polypyrole (Pyrrolidine) on carbon fiber felt electrode by electrochemical polymerization was investigated and characterized by Fourier transform infrared spectroscopy (FTIR) and scanning electron microscope (SEM).The modified carbon fiber felt electrode was applied to Biofilm Electrode Reactor (ber). The effect of electrode modification on the performance of electrode biofilm reactor was investigated and the adhesion of polypyrrole film modified electrode to biofilm was studied.Effects of extracellular polymer composition and biofilm microbial community.The results show that the uniform and stable polypyrrole film can be formed on the surface of carbon fiber electrode by constant voltage electrochemical polymerization with 25 mm AQDS as dopant, and the modification of polypyrrole film on carbon fiber felt electrode can be realized.The modified electrode is applied to the electrode biofilm reactor.The removal efficiency of N03-N in autotrophic denitrification reactor was increased from 67.3% to 83.9% in the control reactor, and the treatment effect was increased by 24.70.The removal rate of COD in the methanogenic reactor was increased by about 18%, and the methane production was increased about 3 times compared with the control reactor.The biofilm of electrode in two kinds of reactors was measured and analyzed by scanning electron microscope (SEM).The results show that the modification of electrode can improve the content of extracellular polymer and the ratio of protein and polysaccharide, thus improving the activity, strength and stability of biofilm.The results of 16s rDNA analysis of biofilm on hydrogen autotrophic denitrifying electrode indicate that the dominant bacteria of polypyrrole coated electrode biofilm are Hydrogenophaga spp and Thauera spspia, which are different from those of control electrode biofilm colony composition.The dominant bacteria were Dechloromonas sp., and its biofilm colony composition was more diversified.It was Methanomicrobia spp (methanogenic microorganism), while the dominant biofilm bacteria in the control group was Methanobacterium spp (methanogenic bacteria), and the composition of the two biofilms was obviously different between the two reactors.This indicates that the modification of electrode material has an effect on the composition of microbial community of electrode biofilm.
【学位授予单位】：大连理工大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：X703

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