莱茵衣藻Chlammydomonas reinhardtii净化酒糟废水研究

发布时间：2018-04-08 12:34

  本文选题：酒糟废水　切入点：莱茵衣藻　出处：《四川师范大学学报(自然科学版)》2016年06期

【摘要】：通过结合废水处理与能源微藻培养,既可以实现废水的无害化处理,也可以为微藻的培养提供营养组分和大量水源.以莱茵衣藻(Chlammydomonas reinhardtii)为实验材料,以酒糟废水为培养基,构建室内微型生态系统,考察了不同处理方法下的酒糟废水接种莱茵衣藻后的总氮(TN)、总磷(TP)、化学需氧量(COD)的去除情况以及微藻蛋白提取情况.结果表明:酒糟废水稀释液初始TN质量浓度位于9.39~30.86 mg·L-1时,废水总氮去除率在70%~80%之间;初始TP质量浓度位于3.45~10.93 mg·L-1时,总磷去除率较高,最高达到86.59%;不同稀释倍数的酒糟废水COD去除率均高于50%.而稀释80倍、50倍以及50倍(氮磷质量浓度比为108∶7)的藻可溶性蛋白增长均比较明显,最大值可达到11.36 mg·L-1,同时也说明通过外加氮、磷调节氮磷的初始质量浓度比至108∶7,可大大提高其产量.总体上,在稀释倍数为50倍(TN、TP初始质量浓度分别为18.23、6.99 mg·L-1)时,莱茵衣藻生长良好,废水中TN、TP、COD去除效果均比较明显,对水质的净化效果最佳,同时可获得较高质量浓度的藻可溶性蛋白,这为酒糟废水与微藻耦合规模化培养提供了一种新的思路.
[Abstract]:By combining wastewater treatment with energy microalgae culture, it can not only realize the harmless treatment of wastewater, but also provide nutrient components and a large amount of water for the cultivation of microalgae.Using Chlammydomonas reinhardtii (Chlammydomonas reinhardtii) as experimental material and distillery wastewater as culture medium, an indoor micro-ecosystem was constructed.The removal of total nitrogen (TNN), total phosphorus (TP) and chemical oxygen demand (COD) from distiller's grains wastewater after inoculation with Chlamydomonas rhinorrhoeae and the extraction of microalgae protein were investigated.The results showed that when the initial TN concentration of distiller's grains wastewater was 9.39 ~ 30.86 mg 路L ~ (-1), the removal rate of total nitrogen was between 70% and 80%, and the total phosphorus removal rate was higher when the initial TP concentration was 3.45 ~ 10.93 mg 路L ~ (-1).The COD removal rate of distiller's grains wastewater with different dilution times was higher than that of 50%.However, 80 times dilution 50 times and 50 times (nitrogen / phosphorus mass concentration ratio of 108: 7) increased the soluble protein obviously, and the maximum value reached 11.36 mg / L, which also indicated that the addition of nitrogen,When phosphorus adjusts the initial mass concentration ratio of nitrogen and phosphorus to 108: 7, the yield can be greatly increased.On the whole, when the dilution multiple was 50 times, the initial concentration of TNN TP was 18.23 ~ 6.99 mg 路L ~ (-1), the growth of Chlamydomonas rhinella was good, and the removal efficiency of TPCOD in wastewater was obvious, and the purification effect was the best for water quality.At the same time, a higher concentration of algae soluble protein can be obtained, which provides a new way for coupling large-scale culture of distiller's grains wastewater and microalgae.
【作者单位】： 四川大学建筑与环境学院;四川大学新能源与低碳技术研究院;成都市环境保护科学研究院固体废物与危险化学品管理研究所;四川师范大学化学与材料科学学院;
【基金】：国家自然科学基金(31100374) 成都市科技局项目(2015-HM01-00013-SF)
【分类号】：X797
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本文编号：1721638