宏基因组方法比较分析深海和珠江口沉积物中抗生素耐药基因的特征

发布时间：2018-04-14 23:37

本文选题：抗生素耐药基因 + 宏基因组分析　；参考：《中山大学学报(自然科学版)》2017年02期

【摘要】：细菌对抗生素的耐药性是全球关注的环境健康问题之一。该研究旨在使用宏基因组方法比较分析西太平洋深海和珠江口沉积物中抗生素耐药基因,认识抗生素使用与环境细菌耐药性间的关系。研究发现,深海沉积物多重耐药基因的含量高达77.8%;珠江口沉积物多重耐药基因只有27.2%,常用抗生素的耐药基因(磺胺类、大环内酯类、氨基糖苷类等)含量明显提高(约70%)。沉积物中共发现45种耐药基因亚型,其中7种基因亚型(acrB、amrB、bacA、ceoB、macB、mexB和smeE)能在所有沉积物中发现。深海沉积物中质粒仅携带0.3%的耐药基因,而珠江口沉积物则超过40%。研究表面,由于珠江口周边区域常用抗生素的广泛使用,其沉积物中细菌抗生素耐药性明显提高、耐药机制趋于多样化。
[Abstract]:Bacterial resistance to antibiotics is one of the global environmental health concerns.The purpose of this study was to compare and analyze antibiotic resistance genes in sediments from the deep sea of the western Pacific Ocean and the Pearl River Estuary by using a macro genome method and to understand the relationship between antibiotic use and environmental bacterial resistance.It is found that the content of multidrug resistance genes in deep-sea sediments is as high as 77.8, and that in sediments of Pearl River Estuary is only 27.2.The content of commonly used antibiotic resistant genes (sulfonamides, macrolides, aminoglycosides, etc.) is obviously increased (about 70%).A total of 45 subtypes of drug resistance genes were found in sediments, of which 7 were found in all sediments.The plasmids contained only 0.3% of drug resistance genes in deep sea sediments, but more than 40% in Pearl River Estuary sediments.Because of the extensive use of common antibiotics in the area around the Pearl River Estuary, the antibiotic resistance of bacteria in the sediments increased obviously, and the mechanism of drug resistance tended to be diversified.
【作者单位】：南方医科大学公共卫生学院;南方医科大学珠江医院;中山大学孙逸仙纪念医院;中山大学海洋学院;
【基金】：广东省自然科学基金(2014A030313195)
【分类号】：X55

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