青霉素对土壤微生物群落结构的影响

发布时间：2018-05-08 03:19

  本文选题：青霉素 + 微生物多样性　； 参考：《生态与农村环境学报》2016年02期

【摘要】：利用Biolog-ECO技术分析不同青霉素浓度处理下土壤微生物的群落代谢活性及结构多样性,利用变性梯度凝胶电泳(denaturing gradient gel electrophoresis,DGGE)技术分析典型微生物群落结构变化情况,探讨青霉素对土壤微生物群落的生态毒理效应。主成分分析(PCA)结果表明,高浓度青霉素(400和800 mg·kg-1)处理能快速诱导土壤中以酚酸类化合物为碳源的微生物菌群富集。DGGE结果显示,在高浓度青霉素(800 mg·kg-1)胁迫初期,土壤中微生物多样性指数(Shannon-Wiener index)显著降低(P0.05);7 d后,主要微生物群落结构随土壤中青霉素降解又逐渐恢复。
[Abstract]:Biolog-ECO technology was used to analyze the metabolic activity and structural diversity of soil microbes under different concentrations of penicillin. The changes of typical microbial community structure were analyzed by denaturing gradient gel electrophoresis (denaturing gradient gel electrophoresis, DGGE), and the ecotoxicological effect of penicillin on soil microbial community was discussed. The results of principal component analysis (PCA) showed that high concentration of penicillin (400 and 800 mg. Kg-1) could quickly induce the enrichment of microbiological bacteria in soil with phenolic acids as carbon sources, and the results showed that the microbiological diversity index (Shannon-Wiener index) in the soil decreased significantly (P0.05) at the early stage of high concentration of penicillin (800 mg. Kg-1). After 7 d, the main microbial community structure gradually recovered with the degradation of penicillin in soil.

【作者单位】： 环境保护部南京环境科学研究所;东南大学能源与环境学院;
【基金】：环保公益性行业科研专项(201209024) 国家自然科学基金青年基金(41401363) 江苏省自然科学基金青年基金(BK20130102) 公益性科研院所基本科研业务费项目
【分类号】：X53;X172
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本文编号：1859733