驯化对餐厨垃圾厌氧消化系统微生物群落结构的影响

发布时间：2018-05-19 23:14

本文选题：驯化 + 厌氧消化　；参考：《环境科学学报》2016年12期

【摘要】：为研究驯化对餐厨垃圾厌氧消化系统微生物群落结构的影响,在单相完全搅拌式(CSTR)反应器内,以农村户用沼气池污泥为接种污泥,进行了餐厨垃圾中温厌氧消化.反应器在3 g·L-1·d-1(以VS计)的负荷下成功启动,并连续45 d维持性能稳定,表明驯化成功.期间采用454焦磷酸测序技术分析了驯化前后系统内的微生物群落结构.结果表明,微生物群落结构与底物密切相关,驯化后细菌及古菌群落都发生明显变化.从细菌群落看,与复杂有机物降解相关的菌类显著下降(如梭菌纲(Clostridia)和(vadin HA17),而易降解碳水化合物发酵菌(如Petrimonas)和脂肪降解菌(如Erysipelotrichia)显著增加.这与餐厨垃圾易降解有机物含量高,且富含淀粉和脂肪相关.丰富的易降解有机物还使得反应器内总挥发性脂肪酸(VFA)浓度((2203±174)mg·L-1)远高于种泥水平((222.0±0.3)mg·L-1),这导致了产甲烷菌由乙酸型的甲烷鬃菌属(Methanosaeta,占85.01%)绝对主导转向氢营养型的甲烷螺菌属(Methanospirillum,占35.35%)、甲烷囊菌属(Methanoculleus,占9.89%)与之(46.97%)共同主导的局面.然而,驯化后Methanosaeta在非最优条件下依然保持主导地位,可见接种污泥的群落组成对厌氧消化系统群落结构的塑造也具有重大影响.
[Abstract]:In order to study the effect of domestication on the microbial community structure in the anaerobic digestion system of food waste, the anaerobic digestion of kitchen waste was carried out in a single phase complete stirred (CSTR) reactor with the sludge of the rural household biogas pool as inoculated sludge. The reactor was successfully started under the load of 3 G. L-1. D-1 (VS), and the performance was stable continuously. During the period of domestication, the microbial community structure in the system was analyzed by 454 pyrosequencing technology. The results showed that the microbial community structure was closely related to the substrate, and the bacteria and the Archaea community were obviously changed after the acclimatization. From the bacterial community, the bacteria related to the degradation of complex organic matter were significantly decreased (such as spindle bacteria. The class (Clostridia) and (vadin HA17), while the easily degraded carbohydrate fermentor (such as Petrimonas) and adipose degrading bacteria (such as Erysipelotrichia) have increased significantly. This is associated with high content of biodegradable organic matter in kitchen waste and rich in starch and fat. The rich and easily degraded organic compounds also make the total volatile fatty acid (VFA) concentration in the reactor (2203 +. 174) mg. L-1) is much higher than the level of mud ((222 + 0.3) mg. L-1), which leads to the absolute dominance of methanogenic bacteria of methanogenic bacteria (Methanosaeta, 85.01%) to the hydrogen nutritive methanomyces (Methanospirillum, 35.35%), methanomyces (Methanoculleus, 9.89%) and (46.97%) Co dominant. However, domestication is domesticated. The post Methanosaeta remains dominant under the non optimal conditions, and the community composition of the inoculated sludge has a significant impact on the construction of the community structure of the anaerobic digestion system.
【作者单位】：重庆大学三峡库区生态环境教育部重点试验室;
【基金】："十一五"国家科技支撑计划重点项目(No.2010BAC67B01) 重庆市科技计划重点项目~~
【分类号】：X799;X172

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