油水淹地石油降解菌群的筛选和鉴定及高效菌群的构建

发布时间：2018-05-27 14:29

  本文选题：油水淹地 + 表面活性剂产生菌　； 参考：《吉林农业大学学报》2016年06期

【摘要】：从油水淹地污染土壤中获得26株石油降解菌,筛选出表面活性剂产生菌1株(H-6)和优势菌6株(H-1、H-17、H-18、H-19、H-20、H-23),结合菌株形态观察、革兰氏染色和16S r DNA序列同源性分析,鉴定表面活性剂产生菌H-6为堀越氏芽孢杆菌(Bacillus horikoshii)。以表面活性剂产生菌H-6为中心,再任选3株优势菌株构建石油降解菌群,得到高效石油降解菌群C5(H-1、H-6、H-18、H-19),通过正交试验得到菌群C5各菌种的最佳接种量。结果表明:接种量15%(H-1)、15%(H-6)、20%(H-18)、10%(H-19),温度25℃,石油含量2 000 mg/L条件下,7 d时石油降解率达到78.87%。这说明高效降解菌群C5对石油具有较好的降解效果,可应用于油水淹地污染土壤的修复。
[Abstract]:26 strains of petroleum degrading bacteria were obtained from contaminated soil under oil flooding, and 1 strain of surfactant producing strain H-6) and 6 strains of dominant bacteria were selected. Combined with morphological observation of strain, Gram staining and homology analysis of 16s r DNA sequence were carried out. The surfactant producing strain H-6 was identified as Bacillus horikoshiiformis. Taking the surfactant producing strain H-6 as the center, 3 dominant strains were selected to construct the petroleum degrading bacteria, and the high-efficiency petroleum degrading bacteria C5H-1H-6H-6H-18H-19 were obtained. The optimum inoculation amount of C5 strains was obtained by orthogonal test. The results showed that the oil degradation rate reached 78.87 at the temperature of 25 鈩，

本文编号：1942484