不同生物处理工艺降解低浓度头孢呋辛酯的机理和抗性基因研究
发布时间:2018-06-06 18:39
本文选题:PPCPs + 传统活性污泥法 ; 参考:《北京化工大学》2015年硕士论文
【摘要】:药品和个人护理用品(PPCPs)是指在环境中持续存在的低浓度有机污染物,对环境带来了很多不利影响。本论文主要研究了三种常用生物处理工艺CAS、SBR和SBBR,对模拟城市生活污水中低浓度头孢呋辛酯的降解机理和污泥中的抗性基因情况,得到结论如下:1.CAS工艺稳定运行条件为:头孢呋辛酯浓度0.5mg/L,污泥负荷0.4kgCOD/(kgMLSS·d),水力停留时间12h,在此条件下,CAS工艺对模拟城市生活污水中COD的去除率为95.82%,对氨氮的去除率为92.41%,对头孢呋辛酯的去除率为91.54%;SBR工艺稳定运行条件为:头孢呋辛酯浓度0.5mg/L,污泥负荷0.3kgCOD/(kgMLSS·d),水力停留时间12h,进水pH 7.5,在此条件下,SBR工艺对模拟城市生活污水中COD的去除率为98.80%,对氨氮的去除率为85.19%,对头孢呋辛酯的去除率为98.17%,并且SBR工艺降解头孢呋辛酯抗生素最符合一级动力学规律;SBBR工艺稳定运行条件为:头孢呋辛酯浓度0.5mg/L,污泥负荷0.4kgCOD/(kgMLSS·d),水力停留时间12h,进水pH为7.5,在此条件下,SBBR工艺对模拟城市生活污水中COD的去除率为94.26%,对氨氮的去除率为58.31%,对头孢呋辛酯的去除率为64.74%,并且SBBR工艺降解头孢呋辛酯抗生素最符合零级动力学规律。2.活性污泥工艺和SBBR工艺降解头孢呋辛酯的中间产物与最终产物类似,都是头孢呋辛酯分子逐渐脱去支链的小分子,最终p-内酰胺环断裂,并且被降解为H20和CO2。3.三种样品的微生物群落多样性顺序为:活性污泥工艺接种污泥序批式生物膜反应器。活性污泥反应器中筛选分离得到苍白杆菌属(Ochrobactrum sp.) CS2和产碱杆菌属(Alcaligenes sp.) CS4两种优势菌株;SBBR反应器中筛选分离得到气单胞菌属(Aeromonas sp.)的BB3和BB4两种优势菌株。4.接种污泥、活性污泥工艺和序批式生物膜反应器(SBBR)污泥中检测出3种β-内酰胺抗性基因:OXA-1、OXA-2和OXA-10。
[Abstract]:Drugs and personal care products (PPCPs) are low concentrations of organic pollutants that persist in the environment and have a lot of adverse effects on the environment. In this paper, the degradation mechanism of low concentration cefuroxime in simulated municipal sewage and the resistance genes in sludge were studied. The results are as follows: 1. The stable operation conditions of CAS process are as follows: cefuroxime concentration 0.5 mg / L, sludge loading 0.4 kg COD / kg MLSs dl, HRT 12 h. Under this condition, the COD removal rate and ammonia nitrogen removal rate of CAS process are 95.82 and 95.82 respectively. 92.41, the removal rate of cefuroxime octyl ester is 91.54 and the stable operating conditions of SBR process are as follows: cefuroxime octyl ester concentration 0.5 mg / L, sludge loading 0.3 kg COD / kg MLSs dl, HRT 12 h, influent pH 7.5. Under these conditions, SBR process can remove COD from simulated municipal sewage. For 98.80, the removal rate of ammonia nitrogen was 85.19, the removal rate of cefuroxime ester was 98.17.The degradation of cefuroxime ester antibiotics by SBR process was most consistent with the first-order kinetic law. The stable operation conditions of SBBR process were as follows: cefuroxime octyl ester concentration 0.5 mg / L, sludge negative. Under these conditions, the removal rate of COD, ammonia nitrogen, cefuroxime ester and cefuroxime were 94.26, 58.31, 64.74, respectively, and the SBBR process was used to degrade cefuroxime, respectively, under the condition that the HRT was 12h and the influent pH was 7.5. The removal rate of COD, ammonia and cefuroxime was 94.26, 58.31 and 64.74 respectively, and the SBBR process was used to degrade cefuroxime. Octyl ester antibiotics accord with the zero order kinetic law. 2. The intermediate products of cefuroxime ester degradation by activated sludge process and SBBR process are similar to the final products. They are small molecules of cefuroxime ester gradually removed from the branched chain, and finally the p-lactam ring breaks down and is degraded into H20 and CO2.3. The sequence of microbial community diversity of the three samples was as follows: activated sludge process inoculated sludge sequencing batch biofilm reactor. Ochrobactrum sp. was isolated from activated sludge reactor. CS2 and Alcaligenes sp. Aeromonas sp. was isolated from two dominant strains of CS4 in SBBR reactor. Two dominant strains of BB3 and BB4. Three 尾 -lactam resistance genes, 1: OXA-1, OXA-2 and OXA-10, were detected in sludge from inoculated sludge, activated sludge process and sequencing batch biofilm reactor (SBBRR).
【学位授予单位】:北京化工大学
【学位级别】:硕士
【学位授予年份】:2015
【分类号】:X703
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