海带内非蛋白巯基化合物的检测分析及重金属诱导转化研究

发布时间：2018-07-03 19:57

本文选题：重金属 + Cd元素　；参考：《中国地质大学(北京)》2015年硕士论文

【摘要】：重金属污染已经日趋成为影响全球的重大生态问题。重金属元素水溶性强,毒性大,可以通过不同途径进入食物链并积累,最后对人类健康造成巨大危害。利用藻类修复是一种较为新颖的治理环境重金属污染的方法,具有其他方法不具备的优点,如高效、环保、成本低等。藻类可以通过表面吸附以及体内络合两个步骤,减少环境中的重金属含量,消除或降低重金属对生物的的毒性。本文实验在实验室条件下,采用3种重金属元素(Zn Cl2、Cu Cl2和Cd Cl2)设置梯度浓度(0.001mmol/L、0.01mmol/L、0.1mmol/L)培养海带。在第1d、3d、5d、7d和10d收集海带样品,同时设置空白对照组海带。首先研究海带对重金属元素的富集规律,然后以Cd元素诱导的海带样品为例,研究海带体内谷胱甘肽GSH和植物络合素PCn的数量变化和相互转化关系。主要获得结论如下:1.海带对重金属有较强的富集能力,诱导时间增加,富集量增加,富集倍数变大;重金属浓度增加,富集量增加,富集倍数却变小。2.Cd元素诱导浓度增加,海带中GSH的含量增加,但增加趋势变缓。GSH含量也随着胁迫时间的增长而增加。但不同组相关性系数差异较大。3.在海带样品中分离检测到4中非蛋白巯基化合物,分别为GSH、PC2、PC3、PC4,同时测定了非蛋白巯基化合物总量。加入重金属离子后,海带藻体内非蛋白巯基化合物含量明显增多,后期呈现下降趋势,并且与Cd元素呈现正相关关系。GSH含量迅速增多,3d之后又大幅下降。推测GSH主要消耗于PCn化合物的转化合成。GSH占非蛋白巯基化合物的巯基比例最大。在检测到的PCn化合物中,PC2在对照组和诱导组中均存在,说明应对重金属离子时,PC2的灵敏度最高。PC3在非蛋白巯基化合物中所占比例最大。PC4被检测到最少。
[Abstract]:Heavy metal pollution has increasingly become a major ecological problem affecting the world. Heavy metal elements are water-soluble and toxic, which can enter the food chain and accumulate through different ways, and finally cause great harm to human health. Phytoremediation is a novel method to control heavy metal pollution in the environment, which has many advantages, such as high efficiency, environmental protection, low cost and so on. Algae can reduce the content of heavy metals in the environment and eliminate or reduce the toxicity of heavy metals to organisms by two steps of surface adsorption and in vivo complexation. In this paper, three heavy metal elements (ZnCl _ 2Cu _ Cl _ 2 and CD _ Cl _ 2) were used to culture kelp (0.001mmol / L ~ 0.01mmol / L ~ (0.1) mmol 路L ~ (-1) with three heavy metals (Zn _ (Cl _ 2) Cu _ (Cl _ 2) and CD _ (Cl _ 2) in laboratory. The kelp samples were collected on the 1st day 3 d ~ 5 d and 10 d, and a blank control group was set up at the same time. The enrichment of heavy metal elements in Laminaria japonica was studied at first, and then the quantity of glutathione GSH and the relationship between glutathione GSH and plant complex PCN in Laminaria japonica were studied by taking the sample of kelp induced by CD as an example. The main conclusions are as follows: 1. Laminaria japonica has a strong enrichment ability to heavy metals, the induction time increases, the enrichment amount increases, the enrichment multiple increases, the heavy metal concentration increases, the enrichment multiple increases, the concentration multiple decreases, and the GSH content in Laminaria japonica increases. However, the increasing trend of GSH also increased with the increase of stress time. But the correlation coefficient of different groups is different. 3. Four non-protein sulfhydryl compounds were isolated and detected in kelp samples, which were GSHHPC2PC3PC4, and the total amount of non-protein sulfhydryl compounds was determined at the same time. After the addition of heavy metal ions, the content of non-protein sulfhydryl compounds in Laminaria significantly increased, and decreased in the later stage, and showed a positive correlation with CD. GSH content increased rapidly for 3 days and then decreased significantly. It is assumed that GSH is mainly consumed in the conversion synthesis of PCN compounds. GSH accounts for the largest proportion of sulfhydryl groups in nonprotein sulfhydryl compounds. PC2 was detected in both the control and induction groups, indicating that the sensitivity of PC2 was the highest. PC3 accounted for the largest proportion of non-protein sulfhydryl compounds. PC4 was the least detectable.
【学位授予单位】：中国地质大学(北京)
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：X835

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