还原氧化石墨烯生物制备及其促进生物还原硝基苯研究

发布时间：2018-07-06 12:34

本文选题：还原氧化石墨烯 + 醌类化合物　；参考：《大连理工大学》2015年硕士论文

【摘要】：石墨烯是由单层碳原子构成的新型二维碳纳米材料,在电学、力学和热学等方面具有优异的性能,其制备方法和应用成为当今研究热点。随着其生产和使用量的不断增加,石墨烯材料不可避免的通过各种途径释放到环境中,给生态环境带来风险。近年来,人们对于石墨烯材料在多孔介质中的迁移及其对污染物的迁移归趋的影响进行了研究,但有关石墨烯材料与微生物相互作用的报道较少。本论文主要针对醌类化合物对生物还原氧化石墨烯反应活性的影响和石墨烯材料对生物转化污染物的影响进行研究。考察蒽醌-2,6-二磺酸钠(AQDS)、葱醌-2-磺酸钠(AQS)、蒽醌-2-羧酸(AQC)和5-羟基-1,4-萘醌(JQ)对Shewanella oneidensis MR-1还原氧化石墨烯(GO)的影响。实验结果表明,AQS. AQDS和JQ对S. oneidensis MR-1还原GO起到促进作用,AQC反而起抑制作用,可能源于其对细菌的生物毒性。AQS介导生物制备的还原氧化石墨烯(Bio-rGO)具有过氧化物模拟酶的催化活性,可以催化H2O2对3,3’,5,5’-四甲基联苯胺(TMB)的氧化反应,且酶反应动力学符合米门方程。推测Bio-rGO的催化机理为Bio-rGO促进电子由TMB向H202传递,从而加速H202对TMB的氧化反应。基于Bio-rGO的过氧化物模拟酶性质,建立了一种快速测定葡萄糖的比色检测方法。该检测方法的线性范围为1～120μM,检测限为1μM,具有良好的特异性。考察还原氧化石墨烯(rGO)、氮掺杂还原氧化石墨烯(N-rGO)、负载金属纳米金还原氧化石墨烯(Au-rGO)和氨基-甲酰基咪唑修饰还原氧化石墨烯(imidazole-rGO)对S. oneidensis MR-1还原硝基苯的影响。实验结果表明,4种石墨烯材料均可以促进S. oneidensis MR-1还原硝基苯,促进效果由高到低的顺序为N-rGOrGOAu-rGO imidazole-rGO。通过对石墨烯材料的表征分析发现,石墨烯材料的比表面积和电化学活性是影响其对生物还原硝基苯促进效果的主要因素。
[Abstract]:Graphene is a new two-dimensional carbon nanomaterial composed of monolayer carbon atoms. It has excellent properties in electrical, mechanical and thermal fields. With the increasing of its production and usage, graphene materials are inevitably released into the environment through various ways, which brings risks to the ecological environment. In recent years, the migration of graphene materials in porous media and their effects on the migration and fate of pollutants have been studied, but there are few reports on the interaction between graphene materials and microorganisms. In this paper, the effects of quinones on the activity of bioreoxidized graphene and the effects of graphene materials on bioconversion pollutants were studied. The effects of sodium anthraquinone (AQDS), onion quinone sodium disulfonate (AQS), anthraquinone 2-carboxylic acid (AQC) and 5-hydroxy-1-anaphthoquinone (JQ) on the reduction of graphene oxide (go) in Shewanella oneidensis MR-1 were investigated. The experimental results show that the aqua. AQDS and JQ promote the reduction of go by S.AQC, which may be due to its biological toxicity to bacteria. The reductive graphene (Bio-rGO) mediated by AQS has the catalytic activity of peroxide-mimic enzyme. It can catalyze the oxidation of TMB by H _ 2O _ 2, and the kinetics of enzyme reaction accords with the equation of rice gate. The catalytic mechanism of Bio-rGO is that Bio-rGO promotes electron transport from TMB to H202, thus accelerates the oxidation of TMB by H202. Based on the properties of Bio-rGO peroxidase, a rapid colorimetric method for the determination of glucose was established. The linear range and detection limit of this method are 1 ~ 120 渭 m and 1 渭 m respectively. The effects of reduced graphene oxide (RGO), nitrogen-doped reductive graphene (N-rGO), supported nano-gold reductive graphene (Au-rGO) and imidazole-rgo (imidazole-rgo) on the reduction of nitrobenzene were investigated. The experimental results show that all four graphene materials can promote the reduction of nitrobenzene by S.oneidensis MR-1 in the order of N-rGOrGOAu-rgo imidazole-rgo. It is found that the specific surface area and electrochemical activity of graphene materials are the main factors that influence the promotion of bioreduction of nitrobenzene.
【学位授予单位】：大连理工大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：X132

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