多壁碳纳米管对水稻幼苗的植物毒性研究
发布时间：2018-07-13 10:03
【摘要】：考察了浓度为10、50和100 mg·L~(-1)的多壁碳纳米管(MWCNTs)的植物毒性,通过测量水稻生长指数、根系氧化应激反应、细胞膜损伤和生理功能的变化,探索了其具体的毒性机制。将水稻幼苗暴露于不同浓度的MWCNTs悬浮液中培养10 d后,高浓度处理组(100 mg·L~(-1))中水稻幼苗地上部分和根系鲜重分别降低至对照组的87.6%±1.1%和69.2%±7.8%。对根系深入研究发现氧化应激反应和细胞膜的损伤主要出现在高浓度处理组,此时过氧化氢酶(CAT)活性由对照组的(8.8±1.6)U·mg-1prot(protein,蛋白质)增加至(16.3±2.8)U·mg-1prot,丙二醛(MDA)含量由对照组的(8.0±0.3)μmol·g-1FW(fresh weight,鲜重)增加至(15.1±1.4)μmol·g-1FW。然而,水稻根系的生化酶活性在低浓度(10 mg·L~(-1))时就开始明显降低。通过透射电镜(TEM)观察发现,MWCNTs颗粒分布在水稻幼苗根系细胞内,从而证实了MWCNTs能被植物细胞吸收。
[Abstract]:The phytotoxicity of multiwalled carbon nanotubes (MWCNTs) with concentrations of 1050mg / L and 100mg / L ~ (-1) was investigated. The specific toxicity mechanism of MWCNTs was explored by measuring the growth index, root oxidative stress response, cell membrane damage and physiological function changes. After the rice seedlings were exposed to different concentrations of MWCNTs suspension for 10 days, the aboveground part and root fresh weight of rice seedlings in the high concentration treatment group (100 mg L ~ (-1) decreased to 87.6% 卤1.1% and 69.2% 卤7.8% of the control group, respectively. It was found that oxidative stress response and cell membrane damage mainly occurred in the high concentration treatment group. The activity of catalase (cat) increased from (8.8 卤1.6) U mg-1prot (protein) to (16.3 卤2.8) U mg-1prot. the content of malondialdehyde (MDA) increased from (8.0 卤0.3) 渭 mol g -1 FW (fresh weight, fresh weight to (15.1 卤1.4) 渭 mol g -1 FW. However, the biochemical enzyme activity of rice roots began to decrease at low concentration (10 mg / L ~ (-1). Transmission electron microscopy (TEM) showed that MWCNTs particles were distributed in root cells of rice seedlings, which proved that MWCNTs could be absorbed by plant cells.
【作者单位】： 湖南大学环境科学与工程学院;湖南大学环境生物与控制教育部重点实验室;
【基金】：湖南大学交叉学科项目(531107040761) 国家自然科学基金(51579095)
【分类号】：X173
[Abstract]:The phytotoxicity of multiwalled carbon nanotubes (MWCNTs) with concentrations of 1050mg / L and 100mg / L ~ (-1) was investigated. The specific toxicity mechanism of MWCNTs was explored by measuring the growth index, root oxidative stress response, cell membrane damage and physiological function changes. After the rice seedlings were exposed to different concentrations of MWCNTs suspension for 10 days, the aboveground part and root fresh weight of rice seedlings in the high concentration treatment group (100 mg L ~ (-1) decreased to 87.6% 卤1.1% and 69.2% 卤7.8% of the control group, respectively. It was found that oxidative stress response and cell membrane damage mainly occurred in the high concentration treatment group. The activity of catalase (cat) increased from (8.8 卤1.6) U mg-1prot (protein) to (16.3 卤2.8) U mg-1prot. the content of malondialdehyde (MDA) increased from (8.0 卤0.3) 渭 mol g -1 FW (fresh weight, fresh weight to (15.1 卤1.4) 渭 mol g -1 FW. However, the biochemical enzyme activity of rice roots began to decrease at low concentration (10 mg / L ~ (-1). Transmission electron microscopy (TEM) showed that MWCNTs particles were distributed in root cells of rice seedlings, which proved that MWCNTs could be absorbed by plant cells.
【作者单位】： 湖南大学环境科学与工程学院;湖南大学环境生物与控制教育部重点实验室;
【基金】：湖南大学交叉学科项目(531107040761) 国家自然科学基金(51579095)
【分类号】：X173
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