吡啶硫酮金属对华美盘管虫抗氧化性的影响

发布时间：2018-07-13 12:52

【摘要】：我国近岸局部海域的重金属污染严重威胁着生态环境和人类健康。吡啶硫酮铜(CuPT)和吡啶硫酮锌(ZnPT)在海洋防污和化工产品中的应用近来逐年增加,对其生态毒性及其机理进行研究迫在眉睫。本文以我国南海常见多毛类——华美盘管虫(Hydroides elegans Haswell)为受试生物,研究了CuPT和ZnPT对华美盘管虫的抗氧化系统的影响。试验结果表明在CuPT和ZnPT胁迫下,华美盘管虫体内的超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽还原酶(GR)的活性,以及还原型谷胱甘肽含量(GSH)和丙二醛(MDA)含量发生与暴露毒物种类、浓度和时间相关的变化。SOD、CAT和GR的活性在较低浓度(25μg·L~(-1))的CuPT胁迫6 h后呈显著上升趋势,但随暴露时间延长(12 h和24 h),GR的活性显著下降;而暴露于较低浓度(50μg·L~(-1))的Zn PT中,仅SOD活性显著升高,CAT和GR的活性都显著下降。在较高Cu PT(100μg·L~(-1))或Zn PT(200μg·L~(-1))浓度中,CAT和GR的活性都显著下降。GSH含量对Cu PT或Zn PT胁迫的响应呈现明显的时间效应,即随暴露时间的延长,通常由显著上升转为显著下降。MDA含量在Cu PT(100μg·L~(-1))或Zn PT(200μg·L~(-1))高浓度组中呈显著升高趋势。
[Abstract]:Heavy metal pollution in coastal areas of China is a serious threat to the ecological environment and human health. The application of copper pyridine thione (CuPT) and zinc pyridine thione (ZnPT) in marine antifouling and chemical products has been increasing year by year. It is urgent to study the ecological toxicity and its mechanism of copper pyridine thione (CuPT) and zinc pyridine thione (ZnPT). In this paper, the effects of CuPT and ZnPT on the antioxidant system of the common polychaete, Hydroides elegans Haswell, in the South China Sea (SCS) were studied. The results showed that the activities of superoxide dismutase (SOD), catalase (cat) and glutathione reductase (gr) were observed under CuPT and ZnPT stress. The contents of reduced glutathione (GSH) and malondialdehyde (MDA) were related to the type, concentration and time of exposure. The activities of cat and gr increased significantly after 6 h of low concentration (25 渭 g / L ~ (-1) of CuPT. However, with the prolongation of exposure time (12 h and 24 h), the activity of gr decreased significantly, but the activities of cat and gr decreased significantly when exposed to Zn PT at lower concentration (50 渭 g / L ~ (-1). The activities of cat and gr decreased significantly at higher Cu PT (100 渭 g L ~ (-1) or Zn PT (200 渭 g L ~ (-1) concentrations. The content of MDA increased significantly in the groups of Cu PT (100 渭 g L ~ (-1) or Zn PT (200 渭 g L ~ (-1).
【作者单位】：海南大学材料与化工学院;海南大学环境与植物保护学院;海南大学农学院;
【基金】：国家自然科学基金项目(31160098,31360105,31660128) 海南省自然科学基金项目(313104) 海南大学博士科研启动基金项目(kyqd1046)
【分类号】：X171.5;X55

【相似文献】