酪氨酸酶固定化碳材料对苯酚的生物降解性能

发布时间：2018-07-17 20:33

【摘要】：以新型碳材料氧化石墨烯作为载体,利用共价结合方法制备酪氨酸酶固定化氧化石墨烯复合物,并利用该复合物对苯酚进行催化降解,探讨酶的加载特性和酶的催化活性,以及固定化酪氨酸酶催化降解苯酚的最优条件及储存稳定性。通过对固定化酪氨酸酶进行活性和固定量分析后认为:单位质量载体的酶固定量为1.78 mg/mg,单位质量载体的酶活性为1 880.6 U/mg;固定化酪氨酸酶在30h内对47.06mg/L苯酚的降解率可达86.3%,降解反应的最优条件为pH=7.0、温度=25℃;固定化酪氨酸酶在4℃条件下30d后仍保持初始活性的77.7%,其稳定性优于游离酪氨酸酶。另外,在氧化石墨烯上引入磁颗粒,既简化了酶固定流程,又能做到回收利用。
[Abstract]:The graphene oxide complex immobilized by tyrosinase was prepared by covalent binding method with a new carbon material graphene oxide as the carrier. The complex was used to catalyze the degradation of phenol. The loading characteristics and catalytic activity of the enzyme were discussed. The optimal conditions and storage stability of phenol degradation catalyzed by immobilized tyrosinase were also discussed. After quantitative analysis of immobilized tyrosinase activity and fixation analysis, it is concluded that the immobilized amount of the immobilized tyrosinase is 1.78 mg / mgand the enzyme activity of the unit mass carrier is 1 880.6 Umg.The degradation rate of phenol by immobilized tyrosinase within 30 hours is 47.06 mg / L phenol. The optimum conditions of degradation reaction were pH 7.0 and temperature 25 鈩，

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