扑草净降解菌DY-1的筛
发布时间：2018-08-26 21:43
【摘要】：扑草净(Prometryn),又称扑蔓尽、割草佳,1959年由瑞士嘉基(Geigy)公司创制。化学名称为2-甲硫基-4,6二(异丙胺基)-1,3,5-三氮苯,属于均三氮苯类选择性除草剂。扑草净是均三嗪类除草剂最具有代表性的农药之一。扑草净结构稳定,一般条件下不会发生水解、光解以及自然降解,残效时间较长,在自然界中能够持久存在。随着扑草净的大量使用以及使用范围的扩大,扑草净会随降雨或地表水淋冲或植物根系的渗透作用,迁移至地下水,并在水体中富集,通过食物链的富集进一步转移给动植物、水体生物并带来危害,再通过动物迁徙和捕食继续迁移转化,最终影响人类的健康。本实验采用富集培养法,从绥化市北林区长期施用扑草净的水稻田中筛选出一株能够高效降解扑草净的菌株,命名为DY-1。该菌株能够以扑草净作为唯一碳源生长,通过高效液相色谱检测DY-1可在48 h内将培养基中浓度为50 mg/L的扑草净降解,降解率为99.1%,降解效果较为显著。菌株在生长48 h后,通过改变某一因素的数值(温度、pH或菌液初始浓度)并且保证另外两个因素不变,对其进行OD600值的测定,确定降解菌DY-1的最适生长条件:温度为30℃,pH为7.0,菌液初始浓度为接种量的3%-5%。其最适碳源为蔗糖,最适氮源为硝酸铵。通过对降解菌DY-1的形态观察,生理生化特征以及16S r DNA序列同源性结果分析,鉴定该菌株为假单胞菌(Pseudomonas sp.),将降解菌DY-1的16S rDNA基因序列在Genbank中注册,登录号为KU054384。该研究为土壤中扑草净的生物降解以及受扑草净污染土壤的生物修复提供了理论依据。
[Abstract]:Puccinia (Prometryn), also known as fluttering, mowing grass, set up in 1959 by (Geigy), Switzerland. The chemical name is 2-methylthio -4N 6 bis (isopropylamino) -1N 3N 3-triazobenzene, which belongs to the selective herbicide of m-triazobenzene. Propofol is one of the most representative pesticides of triazine herbicides. The structure of Puccinia mongolicum is stable, and the hydrolysis, photolysis and natural degradation will not take place under general conditions. The residual effect is longer, and it can exist in nature for a long time. With the extensive use and the expansion of its scope of use, Puccinomium can migrate to groundwater with rainfall or surface water leaching or the infiltration of plant roots, and is enriched in water bodies and further transferred to plants and animals through enrichment in the food chain, Water organisms bring harm, and then through the migration of animals and predators continue to migrate and transform, and ultimately affect the health of human beings. By using enrichment culture method, a strain which can degrade Pucalyx DY-1. was selected from the paddy field in the north forest area of Suihua city for a long time. The strain was able to grow with propofol as the sole carbon source. DY-1 could be degraded in culture medium with concentration of 50 mg/L within 48 h by HPLC. The degradation rate was 99.1, and the degradation effect was remarkable. After 48 hours of growth, the OD600 value of the strain was determined by changing the value of one factor (temperature pH or initial concentration of the liquid) and ensuring that the other two factors remained unchanged. The optimum growth conditions were determined as follows: the temperature was 30 鈩，
本文编号：2206246
[Abstract]:Puccinia (Prometryn), also known as fluttering, mowing grass, set up in 1959 by (Geigy), Switzerland. The chemical name is 2-methylthio -4N 6 bis (isopropylamino) -1N 3N 3-triazobenzene, which belongs to the selective herbicide of m-triazobenzene. Propofol is one of the most representative pesticides of triazine herbicides. The structure of Puccinia mongolicum is stable, and the hydrolysis, photolysis and natural degradation will not take place under general conditions. The residual effect is longer, and it can exist in nature for a long time. With the extensive use and the expansion of its scope of use, Puccinomium can migrate to groundwater with rainfall or surface water leaching or the infiltration of plant roots, and is enriched in water bodies and further transferred to plants and animals through enrichment in the food chain, Water organisms bring harm, and then through the migration of animals and predators continue to migrate and transform, and ultimately affect the health of human beings. By using enrichment culture method, a strain which can degrade Pucalyx DY-1. was selected from the paddy field in the north forest area of Suihua city for a long time. The strain was able to grow with propofol as the sole carbon source. DY-1 could be degraded in culture medium with concentration of 50 mg/L within 48 h by HPLC. The degradation rate was 99.1, and the degradation effect was remarkable. After 48 hours of growth, the OD600 value of the strain was determined by changing the value of one factor (temperature pH or initial concentration of the liquid) and ensuring that the other two factors remained unchanged. The optimum growth conditions were determined as follows: the temperature was 30 鈩，
本文编号：2206246
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