一株铁还原菌的分离及其利用特性研究
发布时间：2018-08-30 13:01
【摘要】：铁生物异化还原作用是铁地球化学循环过程中的重要环节,因其对环境中的重金属及难降解有机污染物具有良好的固定和降解能力而受到了广泛的关注。开展铁还原菌分离及其特性研究将有助于深入了解微生物铁还原过程,不仅可以丰富污染修复的生物材料,而且为污染修复应用提供重要的理论依据。本研究从污水处理厂的污泥中分离纯化得到高效的铁还原菌,并研究纯培养条件下铁还原菌在不同碳源体系中的铁还原能力、对不同Fe(Ⅲ)的利用能力和电子穿梭体对铁还原过程的影响,并初步研究该铁还原菌共代谢降解三氯乙烯的能力。主要研究成果如下：分离获得的铁还原菌为短杆状革兰氏阴性菌,其最佳的生长条件为pH7,温度35℃、黑暗条件。异化铁生物还原过程的最佳碳浓度为1.5mol/L,蔗糖和葡萄糖是研究菌株的最佳碳源,其后依次是丙酮酸钠、乙酸钠和乳酸钠。蔗糖和葡萄糖为碳源时,Fe(Ⅲ)还原率分别为81%和57.2%。碳源明显影响着异化铁还原过程中的脱氢酶活性、pH和H2分压。各碳源体系中脱氢酶活性依次为丙酮酸钠蔗糖葡萄糖乙酸钠乳酸钠,体系pH值变化幅度大小依次为乙酸钠乳酸钠丙酮酸钠葡萄糖蔗糖,蔗糖、丙酮酸钠和葡萄糖作为电子供体所产生的H2量远大于乳酸盐和乙酸盐。核黄素(VB2)、钴胺素(VB12)、黄腐酸(FA)和蒽醌-2,6-二磺酸钠(AQDS)这四种电子传递物质中,VB2和VB12仅能作为碳源被铁还原菌利用,充当电子供体。FA和AQDS可作为电子穿梭体明显促进Fe(OH)3的还原,增大铁生物还原过程的还原潜势,加速Fe(Ⅲ)还原速度。分离获得的铁还原菌不能以TCE作为碳源来降解TCE,但以蔗糖为共代谢底物时,在最适蔗糖浓度0.125mol/L时其降解率为40.1%；在共代谢体系中添加Fe(OH)3,TCE降解率提高至48.75%；共代谢体系中同时添加电子受体Fe(OH)3和电子穿梭体FA可以进一步提高TCE降解率。
[Abstract]:Iron biological alienation reduction is an important part in the process of iron geochemical cycle. It has attracted extensive attention because of its good fixation and degradation ability to heavy metals and refractory organic pollutants in the environment. The study on the separation and characteristics of iron reducing bacteria will be helpful to understand the process of iron reduction of microorganisms, which can not only enrich the biological materials for remediation of pollution, but also provide important theoretical basis for the application of remediation of pollution. In this study, high efficiency iron reducing bacteria were isolated and purified from sludge of sewage treatment plant, and the iron reducing ability of iron reducing bacteria in different carbon source systems was studied under pure culture conditions. The utilization ability of different Fe (鈪，
本文编号：2213148
[Abstract]:Iron biological alienation reduction is an important part in the process of iron geochemical cycle. It has attracted extensive attention because of its good fixation and degradation ability to heavy metals and refractory organic pollutants in the environment. The study on the separation and characteristics of iron reducing bacteria will be helpful to understand the process of iron reduction of microorganisms, which can not only enrich the biological materials for remediation of pollution, but also provide important theoretical basis for the application of remediation of pollution. In this study, high efficiency iron reducing bacteria were isolated and purified from sludge of sewage treatment plant, and the iron reducing ability of iron reducing bacteria in different carbon source systems was studied under pure culture conditions. The utilization ability of different Fe (鈪，
本文编号：2213148
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