四环素类抗生素对铜绿微囊藻生理效应研究

发布时间：2018-09-09 19:37

【摘要】：近年来,水生生态系统中检测到有抗生素残留物质存在。这些残留于环境中的抗生素会对水生生物造成潜在的安全威胁。此外,蓝藻水华也已经严重威胁到人类安全用水和环境的安全。然而,有关抗生素对蓝藻水华中优势藻种毒性的研究比较有限。本研究旨在评估三种典型四环素类抗生素(四环素、金霉素和土霉素)对铜绿微囊藻的毒性作用。并且对细胞生长、叶绿素a和蛋白质含量,酶促抗氧化活性以及藻毒素产生规律进行了研究。结果显示,环境中低剂量四环素类抗生素显著抑制细胞生长。铜绿微囊藻在三种四环素类抗生素浓度为0.05mg/L经过9天培养,细胞内叶绿素a和蛋白质含量显著下降。铜绿微囊藻暴露在浓度为0.2到1mg/L的四环素培养基中,超氧化物歧化酶(SOD)活性升高,但过氧化物酶(POD)和过氧化氢酶(CAT)活性下降。与此相反,铜绿微囊藻暴露于土霉素和金霉素浓度0.2到1 mg/L和0.01到0.05 mg/L时,SOD活性降低,但POD和CAT活性增加后。这些实验结果表明,四环素类抗生素影响水华优势藻种铜绿微囊藻细胞生长和蛋白质合成,这些环境浓度下的四环素类抗生素可以诱导铜绿微囊藻产生氧化应激。三种抗生素对铜绿微囊藻中MG-LR的合成明显起促进作用,其中三种抗生素对微囊藻毒素MC-LR合成效果促进程度为四环素最强,土霉素次之,而金霉素最弱。对铜绿微囊藻MC-RR的合成也起促进作用,合成促进效果为土霉素最强,四环素次之,金霉素最弱。因此,本研究可以提供进一步地了解四环素类抗生素和受控使用抗生素的毒害作用,以及认识四环素类抗生素对生态毒理性的影响。
[Abstract]:In recent years, antibiotic residues have been detected in aquatic ecosystems. These antibiotic residues in the environment pose a potential safety threat to aquatic organisms. In addition, cyanobacterial bloom has seriously threatened the safety of water use and the environment. However, the toxicity of antibiotics to dominant algae species in cyanobacterial bloom has been studied. The purpose of this study was to evaluate the toxicity of three typical tetracycline antibiotics (tetracycline, chlortetracycline and oxytetracycline) to Microcystis aeruginosa. Cell growth, chlorophyll a and protein content, enzymatic antioxidant activity and algal toxin production were also studied. Chlorophyll A and protein content in the cells of Microcystis aeruginosa cultured in three tetracycline antibiotic concentrations of 0.05 mg/L for 9 days decreased significantly. Microcystis aeruginosa exposed to tetracycline media with concentrations of 0.2 to 1 mg/L increased the activity of superoxide dismutase (SOD), but the activities of peroxidase (POD) and superoxide dismutase (POD) were increased. On the contrary, the SOD activity of Microcystis aeruginosa decreased when exposed to oxytetracycline and chlortetracycline concentrations of 0.2 to 1 mg/L and 0.01 to 0.05 mg/L, but POD and CAT activity increased. These results showed that tetracycline antibiotics affected the growth and protein synthesis of Dominant Algae Microcystis aeruginosa. Three kinds of antibiotics promoted the synthesis of MG-LR in Microcystis aeruginosa. Among them, three kinds of antibiotics promoted the synthesis of microcystin MC-LR most strongly, oxytetracycline followed by chlortetracycline, and chlortetracycline was the weakest. Therefore, this study can provide a further understanding of the toxic effects of tetracycline antibiotics and controlled antibiotics, as well as the ecological toxicity of tetracycline antibiotics.
【学位授予单位】：上海应用技术学院
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：X52;X173

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