秸秆载体腐解对微生物修复石油污染的影响

发布时间：2018-10-13 19:29

【摘要】：以一种农作物秸秆(简称MG)为载体,采用高效石油烃降解菌群制备固定化微生物,在花盆中模拟石油污染土壤的原位修复。在污染土壤中分别加入秸秆(MG)、游离菌、秸秆(MG)+游离菌、秸秆(MG)固定化微生物,并以只含土著菌的土壤样品为对照,定期测定不同修复方式下土样中石油烃、腐殖质、胡敏酸含量和微生物数量,考察微生物对石油污染土壤的修复作用及MG腐解对修复的影响。结果表明,随着修复的进行,MG在土壤中逐渐腐解,土壤中腐殖质和胡敏酸质量分数明显增高,加入固定化微生物的土样中腐殖质和胡敏酸质量分数增长率最高,分别增加了44.58%和39.27%;加入固定化微生物的土样修复35d的石油烃降解率最高,达到41.78%,且微生物数量最多,其次为添加MG+游离菌的土样,石油烃降解率为31.75%,均高于只含游离菌土样的石油烃降解率27.83%。载体MG腐解产生的腐殖质和胡敏酸对石油污染土壤的修复起到了明显的促进作用。
[Abstract]:The immobilized microorganism was prepared by high efficient petroleum hydrocarbon degrading microflora using a crop straw (MG) as carrier, and in situ remediation of oil-contaminated soil was simulated in flowerpot. The immobilized microorganisms of straw (MG), free bacteria, straw (MG) free bacteria and straw (MG) were added to the polluted soil, and the petroleum hydrocarbon and humus in soil samples under different remediation methods were determined periodically with the control of soil samples containing only indigenous bacteria. The content of Hu Min acid and the quantity of microorganism were studied to investigate the remediation of petroleum contaminated soil by microorganism and the effect of MG decomposition on remediation. The results showed that MG decomposed gradually in soil with remediation, humus and Hu Min acid content in soil increased obviously, humus and Hu Min acid mass fraction in soil samples added immobilized microorganism increased the highest. The degradation rate of petroleum hydrocarbon was the highest (41.78%) in the soil sample with immobilized microorganism for 35 days, and the number of microorganism was the highest, followed by the soil sample supplemented with MG free bacteria, which increased by 44.58% and 39.27% respectively, and the degradation rate of petroleum hydrocarbon was the highest in 35 days after the immobilized microorganism was added. The degradation rate of petroleum hydrocarbon was 31.75, which was higher than that of soil sample containing only free bacteria 27.83%. Humus produced by carrier MG decomposition and Hu Min acid played a significant role in the remediation of petroleum contaminated soil.
【作者单位】：中国石油大学化学工程学院环境与安全工程系;
【基金】：山东省自然科学基金项目(ZR2014BM023) 中国石油科技创新基金项目(2009D-5006-07-01) 青岛市科技发展指导计划项目(KJZD-12-65-jch) 中央高校基本科研业务费专项资金(11CX05011A) 中国石油大学(华东)研究生创新工程(CX-1219)、(CX2013035) 研究生金点子项目(JDZ1405030) 中央高校基本科研业务费专项资金(14CX06101A)资助
【分类号】：X172;X53

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