城市污水户外跑道池培养微藻的生长促进方法研究

发布时间：2018-11-01 21:03

【摘要】：利用城市污水培养产油微藻,在净化污水的同时生产生物柴油,既能缓解日趋严重的能源危机,又能减轻水体污染。微藻培养时提高其产量是整套微藻制备生物柴油工艺能够顺利运行的源头保证,能够有效降低成本。本论文的目的就是在户外利用城市污水培养微藻,寻找出一套能够最大限度地促进微藻生长的优化组合方法,提高微藻生物柴油的经济可行性。本研究通过户外实验发现三种外加刺激方法(磁场、植物激素,细菌)对城市污水培养的蛋白核小球藻的生长,藻干重及油脂产量均有一定的促进作用,得出了每种促进方法使用的最适宜条件。研究了这三种外加刺激方法组合运用效果,通过正交实验确定了户外城市污水培养小球藻促进其生长及油脂积累的最佳组合方式。将最佳组合运用到户外跑道池扩大化装置中,在批次培养小球藻时,最佳组合使得小球藻的OD值提高了8.06%,叶绿素a的浓度提高了43.94%,藻干重提高了17.85%,油脂产量提高了27.54%;在半连续培养时,研究确定了跑道池藻液的最佳更新率为25%,小球藻的稳定油脂产量为0.18 g/L,城市污水的多项污染物浓度均有所下降。本文还对外加刺激对小球藻的生长促进原因进行了初步探讨,外加刺激使得小球藻的光合色素含量增加,提高了小球藻的光合作用,使得小球藻藻细胞中的抗氧化酶活性增加,促进了藻细胞生长,此外磁处理使得培养液的透光率增加,利于微藻吸收光能,光合细菌能改善培养液的p H,有效地改善了小球藻的生长环境,促进小球藻的生长。
[Abstract]:Using municipal sewage to cultivate oil-producing microalgae and produce biodiesel while purifying sewage can not only alleviate the serious energy crisis but also reduce water pollution. Increasing the production of microalgae is the source guarantee that the whole process of microalgae producing biodiesel can run smoothly and can effectively reduce the cost. The purpose of this paper is to cultivate microalgae in outdoor sewage, to find a set of optimal combination method which can promote the growth of microalgae to the maximum extent, and to improve the economic feasibility of microalgae biodiesel. In this study, it was found that three stimulation methods (magnetic field, phytohormone, bacteria) could promote the growth, dry weight and oil production of Chlorella Proteinuca cultured in municipal sewage. The optimum conditions for the use of each method are obtained. The combined effects of these three stimulation methods were studied, and the optimum combination of growth and oil accumulation of Chlorella vulgaris cultured with outdoor municipal sewage was determined by orthogonal experiment. Applying the best combination to the outdoor runway pool expansion device, the best combination increased the OD value, chlorophyll a concentration and dry weight of Chlorella vulgaris by 8.06, 43.94 and 17.85, respectively, in the batch culture of Chlorella vulgaris, which increased the OD value of Chlorella vulgaris by 8.06, the concentration of chlorophyll a by 43.94 and the dry weight of algae by 17.85. Oil yield increased 27.54%; During semi-continuous culture, the optimum rate of upgrading of algae solution in runway was determined to be 25, the stable oil production of Chlorella microphylla was 0.18 g / L, and the concentration of several pollutants in municipal sewage was decreased. The reasons for the growth of Chlorella vulgaris were also discussed. The photosynthetic pigment content of Chlorella vulgaris was increased, the photosynthesis of Chlorella vulgaris was increased, and the antioxidant enzyme activity of Chlorella vulgaris cells was increased. In addition, magnetic treatment increased the transmittance of the medium, which was beneficial to the absorption of light energy by microalgae, and photosynthetic bacteria could improve the growth environment of Chlorella vulgaris and promote the growth of Chlorella vulgaris.
【学位授予单位】：哈尔滨工业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：Q949.2;X703

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