溶液对蛋白质污染膜的清洗效果分析

发布时间：2018-11-03 19:45

【摘要】：采用牛血清蛋白(BSA)代表有机物,自行开发了聚偏氟乙烯(PVDF)膜片,结合耗散型石英晶体微天平(QCM-D),考察不同NaCl浓度条件下BSA在PVDF膜面的微观解吸行为及吸附层结构特征;并使用原子力显微镜测定相应离子条件下与膜污染行为相关的微观作用力大小,综合膜性能恢复实验,解析NaCl溶液对有机物污染膜的清洗机理.结果表明:随着NaCl离子浓度的从1增加到300 mmol·L~(-1),PVDF与羧基官能团之间的相互作用力逐渐减小,BSA污染层松散度逐渐增大;同时引入大量的水合Na~+离子进入BSA污染层内部,破坏BSA污染层内部的静电吸引力和氢键,进而破坏吸附层结构,伴随着BSA污染膜的通量恢复率随着NaCl离子浓度的增加而大幅度提高。
[Abstract]:The polyvinylidene fluoride (PVDF) membrane was developed by using bovine serum protein (BSA) as the representative of organic compounds. The membrane was combined with dissipative quartz crystal microbalance (QCM-D). The microcosmic desorption behavior of BSA on the surface of PVDF membrane and the structural characteristics of the adsorbed layer under different NaCl concentrations were investigated. The microcosmic force related to membrane fouling behavior was determined by atomic force microscope (AFM), and the cleaning mechanism of organic fouling membrane by NaCl solution was analyzed by synthesizing the membrane performance recovery experiment. The results show that with the increase of NaCl ion concentration from 1 to 300 mmol L ~ (-1), PVDF and carboxyl functional group interaction force decreases gradually, and the looseness of BSA pollution layer increases gradually. At the same time, a large number of hydrated Na~ ions were introduced into the BSA polluted layer, which destroyed the electrostatic attraction and hydrogen bond in the BSA polluted layer, and then destroyed the structure of the adsorption layer. With the increase of NaCl ion concentration, the flux recovery rate of the membrane fouled by BSA increased greatly.
【作者单位】：西安建筑科技大学环境与市政工程学院;
【基金】：国家自然科学基金资助项目(51178378,51278408) 陕西省科技创新项目(2012KTCL03-06,2013KTCL03-16) 西安建筑科技大学博士创新基金资助项目(DB03154)
【分类号】：X703

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