耦合法提取剩余污泥中蛋白质及其后续处理研究

发布时间：2018-11-04 16:03

【摘要】：城市污水厂的剩余污泥,不加处理任意排放,会严重的污染环境。从剩余污泥中提取蛋白质,符合污泥资源化的目的,是当前研究的热点课题之一,耦合技术提取剩余污泥中蛋白质具有明显优势。本文以武汉市沙湖污水处理厂剩余污泥为研究对象,探讨了基于热、基于超声波的耦合技术在剩余污泥蛋白质提取上的应用研究,分别从反应体系的温度、反应时间、pH值、超声波输出功率、超声波输出时间、过氧化氢投加量和作用时间、硫酸钠投加量等条件进行系统的分析研究,对今后的剩余污泥蛋白质的提取的研究起到借鉴作用。本研究主要可分为三个部分:基于热耦合工艺的蛋白质提取研究;基于超声波耦合工艺的蛋白质提取研究;基于最佳处理条件下上清液作为污水厂补充碳源的可行性研究。通过研究得出一些新的结论。1)在单一酸解、热解作用下的蛋白质提取效果较差,热解与酸耦合处理技术不仅具有较高的蛋白质提取率,而且试验过程中酸性环境会在体系中保护蛋白质,阻碍美拉德反应的发生,缩短反应时间。在试验温度为110℃,试验pH条件为1左右,处理时间为6h的情况下,蛋白质质提取率达到61.59%,远远大于单一处理条件。2)超声波输出功率为65%(总功率为1200w),超声波处理时间为22min情况下,单一超声波处理的蛋白质提取率为62.28%,提取率较高。单一过氧化氢和超声波耦合过氧化氢处理剩余污泥均可以提取蛋白质,提取率分别为23.56%和59.18%,试验过程中,过氧化氢在水解细胞壁的同时也会对溶液中蛋白质产生影响,而且当试验结束时,溶液中剩余的过氧化氢会继续水解污泥和蛋白质,试验进程较难掌握。而超声波与硫酸钠耦合工艺处理剩余污泥不仅蛋白质提取率高(69.01%),而且试验进程容易掌握,试验结束后无任何因素影响,试验清洁,不需对溶液进行进一步处理,最佳处理条件:超声波输出功率为65%,超声波输出时间为20min,盐(硫酸钠)的投加量为0.7mol/L。3)在最佳污泥处理条件下污泥溶液的上清液可作为碳氮比较低的污水的补充碳源。经超声波耦合盐处理的剩余污泥上清液中含有大量的SCOD、N和P,将处理后的上清液做为营养液培养活性污泥,并对反应器水质情况进行分析。经研究,污泥处理后上清液中SCOD去除率达到68.4%,上清液可作为补充碳源被活性污泥部分利用。
[Abstract]:The excess sludge of the municipal wastewater treatment plant will pollute the environment seriously if it is discharged arbitrarily. The extraction of protein from surplus sludge is in line with the purpose of sludge reuse and is one of the hot topics in current research. The coupling technology has obvious advantages in extracting protein from surplus sludge. In this paper, the application of coupling technology based on heat and ultrasonic in protein extraction of excess sludge from Shahu sewage treatment Plant of Wuhan City was studied. The temperature, reaction time and pH value of the reaction system were studied respectively. The ultrasonic output power, ultrasonic output time, hydrogen peroxide dosage and action time, sodium sulfate dosage and other conditions were systematically analyzed and studied, which could be used for reference in the study of protein extraction from surplus sludge in the future. This study can be divided into three parts: protein extraction based on heat coupling process, protein extraction based on ultrasonic coupling process, and feasibility study of supernatant as carbon source in wastewater treatment plant under optimal treatment conditions. Some new conclusions are obtained through the study. 1) the protein extraction efficiency is poor under the action of single acid hydrolysis and pyrolysis, and the technology of coupling pyrolysis and acid treatment not only has a high protein extraction rate, In addition, acid environment can protect protein in the system, hinder Maillard reaction and shorten reaction time. When the test temperature is 110 鈩，

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