抗锌细菌ZS2的分离鉴定及抗金属特性研究

发布时间：2018-11-23 12:38

【摘要】：筛选可有效降解矿区土壤重金属的抗性细菌,在土壤环境的生物修复中应用前景广阔。从陕西商洛铅锌矿重金属污染土壤中筛选获得一株对重金属锌有高抗性的菌株,命名为ZS2。通过形态特征、生理生化特性及16S r RNA序列分析,初步鉴定该菌为不解糖假苍白杆菌(Pseudochrobactrum asaccharolyticum)。研究其抗重金属及重金属吸附特性,结果表明:ZS2对Zn~(2+)具有较高抗性,最大耐受浓度20 mmol/L,且对多种重金属(Pb~(2+)、Cu~(2+)、Cr6+和Cd~(2+))也具有耐受性;对低浓度Zn~(2+)去除效果最佳,Zn~(2+)浓度为2.0 mmol/L时吸附率高达55.25%;同时对高浓度Zn~(2+)仍具有去除作用。不解糖假苍白杆菌ZS2是鲜有的抗重金属假苍白杆菌属细菌,具有优良的修复土壤重金属污染应用价值。
[Abstract]:Screening resistant bacteria that can effectively degrade heavy metals in mining areas has a bright future in bioremediation of soil environment. A strain with high resistance to heavy metal zinc was obtained from heavy metal contaminated soil of Shangluo lead-zinc mine in Shaanxi Province. It was named ZS2.. By morphological, physiological and biochemical characteristics and 16s r RNA sequence analysis, the strain was identified as (Pseudochrobactrum asaccharolyticum). The results showed that ZS2 had high resistance to Zn~ (2), maximum tolerance concentration of 20 mmol/L, and tolerance to many heavy metals (Pb~ (2), Cu~ (2), Cr6 and Cd~ (2). The removal efficiency of low concentration Zn~ (2) was the best, the adsorption rate of Zn~ (2) was up to 55.25 mmol/L, and the removal of high concentration of Zn~ (2) was still effective. Pseudopallidobacillus saccharum (ZS2) is a rare bacteria resistant to heavy metal, which has good application value in remediation of heavy metal pollution in soil.
【作者单位】： 陕西省微生物研究所微生物资源中心;西安市疾病预防控制中心;西安工程大学环境与化学工程学院;
【基金】：陕西省科学院青年人才培养专项(2014K-34)
【分类号】：X172
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本文编号：2351648