酸性红B高效降解耐盐菌株BY-2的分离鉴定及降解特性研究
发布时间：2019-01-01 07:40
【摘要】：从已运行一年的三相内循环好氧生物流化床系统中分离筛选出一株降解酸性红B的高效耐盐菌株BY-2,通过对菌株的理化形态特征及16s r RNA序列分析,初步确定菌株BY-2为Pseudomonas sp.属。考察了初始pH、盐度、染料浓度以及接种量等条件对酸性红B降解与菌体生长的影响。结果表明:初始pH为7,盐浓度为50 g×L~(-1),酸性红B初始浓度为500 mg×L~(-1),接种量2%,反应18 h时,酸性红B的降解率可达90%以上。降解中间产物的紫外-可见光光谱和GC-MS分析说明菌株BY-2对酸性红B有良好的降解作用,菌株BY-2在实际染料废水处理中具有潜在应用价值。
[Abstract]:A highly salt-tolerant strain BY-2, was isolated from a three-phase aerobic biological fluidized bed system which has been running for one year. The physicochemical and morphological characteristics and 16s r RNA sequence of the strain were analyzed. Preliminary identification of strain BY-2 as Pseudomonas sp. Belong to The effects of initial pH, salinity, dye concentration and inoculation amount on the degradation of acid red B and cell growth were investigated. The results showed that initial pH was 7, salt concentration was 50g 脳 L ~ (-1), initial concentration of acid red B was 500 mg 脳 L ~ (-1). The degradation rate of acid red B was over 90% when inoculation amount was 2 and reaction time was 18 hours. UV-Vis spectra and GC-MS analysis of the intermediate products showed that strain BY-2 could degrade acid red B well, and strain BY-2 had potential application value in the treatment of dye wastewater.
【作者单位】： 北京化工大学化学工程学院;
【基金】：国家科技支撑计划(2011BAE07B09-2)
【分类号】：X172;X788
[Abstract]:A highly salt-tolerant strain BY-2, was isolated from a three-phase aerobic biological fluidized bed system which has been running for one year. The physicochemical and morphological characteristics and 16s r RNA sequence of the strain were analyzed. Preliminary identification of strain BY-2 as Pseudomonas sp. Belong to The effects of initial pH, salinity, dye concentration and inoculation amount on the degradation of acid red B and cell growth were investigated. The results showed that initial pH was 7, salt concentration was 50g 脳 L ~ (-1), initial concentration of acid red B was 500 mg 脳 L ~ (-1). The degradation rate of acid red B was over 90% when inoculation amount was 2 and reaction time was 18 hours. UV-Vis spectra and GC-MS analysis of the intermediate products showed that strain BY-2 could degrade acid red B well, and strain BY-2 had potential application value in the treatment of dye wastewater.
【作者单位】： 北京化工大学化学工程学院;
【基金】：国家科技支撑计划(2011BAE07B09-2)
【分类号】：X172;X788
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