罗红霉素对不同生长期小麦土壤氮形态和脲酶活性的影响

发布时间：2019-01-02 09:20

【摘要】：抗生素类药物作为添加剂被用于养殖业中,大部分以原形或其代谢产物形式进入土壤环境,对土壤环境造成生态危害。通过盆栽实验,研究了不同浓度罗红霉素(ROX)对小麦各生长期根际土壤微生物生物量氮、脲酶和无机氮的影响。结果表明,添加ROX后土壤微生物生物量氮在小麦苗期、拔节期和抽穗期受到显著抑制,而在灌浆期和收获期,土壤微生物生物量氮却显著增长;与对照相比,低浓度(0.2和0.5 mg·kg~(-1))和中浓度(1.0和2.0 mg·kg~(-1))ROX胁迫显著抑制小麦生长苗期、灌浆期和收获期的根际土壤脲酶活性,而在拔节期和抽穗期,却显著诱导脲酶活性;但高浓度(10.0 mg·kg~(-1))ROX胁迫却显著抑制小麦生长期内脲酶活性。低、中浓度ROX胁迫显著诱导小麦生长期中根际土壤中铵态氮浓度增长,而高浓度组ROX胁迫显著抑制土壤中铵态氮浓度。ROX胁迫抑制硝态氮,显示出ROX会影响土壤氮循环。
[Abstract]:Antibiotics are used as additives in aquaculture, most of them enter the soil environment in the form of raw form or its metabolites, which cause ecological harm to the soil environment. The effects of different concentrations of roxithromycin (ROX) on microbial biomass nitrogen urease and inorganic nitrogen in rhizosphere soil of wheat were studied by pot experiment. The results showed that soil microbial biomass nitrogen was significantly inhibited at wheat seedling stage, jointing stage and heading stage after adding ROX, while soil microbial biomass nitrogen increased significantly at filling stage and harvest stage. Compared with the control, low concentration (0. 2 and 0. 5 mg kg~ (-1) and medium concentration (1. 0 and 2. 0 mg kg~) (-1) ROX) significantly inhibited the soil urease activity of wheat at seedling, grain filling and harvest stages. But at jointing stage and heading stage, urease activity was induced significantly. However, high concentration (10.0 mg kg~ (-1) ROX) significantly inhibited urease activity in wheat growth period. Low and medium concentration ROX stress significantly induced the increase of ammonium nitrogen concentration in rhizosphere soil during wheat growth period, while ROX stress significantly inhibited ammonium nitrogen concentration in soil. ROX stress inhibited nitrate nitrogen concentration, indicating that ROX could affect soil nitrogen cycling.
【作者单位】：扬州大学环境科学与工程学院;浙江省植物进化生态学与保护重点实验室;南开大学环境科学与工程学院环境污染过程与基准教育部重点实验室;
【基金】：国家自然科学基金项目(31500425) 江苏省自然科学基金青年科技人才项目(BK20150452) 浙江省植物进化生态学与保护重点实验室开放课题(EEC2014-02)
【分类号】：X53;X171.5

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