氯霉素对费氏弧菌0～24h的Hormesis效应及其机制初探

发布时间：2019-01-24 09:31

【摘要】：为了探究抗生素对发光菌的Hormesis效应,选择费氏弧菌(Vibrio fischeri,V.fischeri)为受试生物,氯霉素为研究对象,测定了0~24h下氯霉素对V.fischeri的发光强度(Hv)及生长量(OD_(600))的作用,探讨氯霉素对V.fischeri的低浓度促进效应及其可能机制.结果表明,氯霉素对V.fischeri的发光强度具有明显的促进作用,且仅出现在细菌生长的延滞期.根据V.fischeri的发光机制,结合Gaussian量子化学计算结果,即氯霉素的最正氢电荷为0.244,远大于烟酰胺腺嘌呤二核苷酸(NADH)的最正氢电荷0.135,提出氯霉素通过提供质子促进荧光反应,进而产生Hormesis效应的可能机制.
[Abstract]:In order to investigate the Hormesis effect of antibiotics on luminescent bacteria, Vibrio flexneri (Vibrio fischeri,V.fischeri) was selected as the tested organism and chloramphenicol was used as the research object. The effects of chloramphenicol on V.fischeri luminescence intensity (Hv) and growth capacity (OD_ (600) at 0 ~ 24 h were measured. The effects of chloramphenicol on the low concentration of V.fischeri and its possible mechanism were discussed. The results showed that chloramphenicol promoted the luminescence intensity of V.fischeri obviously, and only appeared in the lag period of bacterial growth. According to the luminescence mechanism of V.fischeri and the results of Gaussian quantum chemistry calculation, the most positive hydrogen charge of chloramphenicol is 0.244, which is much larger than that of nicotinamide adenine dinucleotide (NADH) 0.135. The possible mechanism of chloramphenicol (chloramphenicol) promoting fluorescence reaction by providing proton to produce Hormesis effect is put forward.
【作者单位】：福州大学材料科学与工程学院;同济大学污染控制与资源化研究国家重点实验室;
【基金】：同济大学污染控制与资源化研究国家重点实验室自主研究项目(PCRRY11003);同济大学英才(攀登)计划(0400219287) 国家自然科学基金(51472050,201177092,21377096,21577105)
【分类号】：X171.5

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