一株异养硝化-好氧反硝化细菌的分离鉴定及其脱氮性能研究

发布时间：2019-02-21 16:50

【摘要】：与传统脱氮菌相比,异养硝化-好氧反硝化菌在脱氮方面具有较大优势并受到广泛关注。以乙酰胺为唯一氮源从活性污泥中分离得到1株脱氮性能较高的异养硝化-好氧反硝化细菌,命名为Y1。经形态观察、生理生化特征和16S rRNA分析后鉴定为Acinetobaterjohnsonii(约氏不动杆菌),革兰氏染色结果为阴性。对Y1菌株进行生理生化鉴定试验,结果显示Y1对吲哚、柠檬酸盐、硫化氢和接触酶的反应呈阳性,表明该菌株能良好的利用以上物质;而甲基红、葡萄糖发酵、蔗糖发酵、明胶液化、淀粉水解、氧化酶、尿素酶试验结果呈阴性,表明该菌株不能很好的利用以上物质。为了检测Y1菌株的脱氮性能,将其分别置于异养硝化培养基和好氧反硝化培养基进行培养,在108 h内,接种Y1菌株的异养硝化培养基中的氨氮去除率约为66.9%,去除速率达0.53 mg.L~(-1)·h~(-1),硝氮去除率约为100%,去除速率达0.10 mg·L~(-1)·h~(-1);在84 h内,接种Y1菌株的好氧反硝化培养基中的硝氮去除率约为69.7%,去除速率达0.74 mg·L~(-1)·h~(-1),上述结果表明Y1菌株的脱氮性能较高。为了进一步研究该菌株的生长需求,保持其它条件不变的情况下,将其分别置于不同碳源和氮源下进行培养,结果表明,菌株Y1在琥珀酸钠为唯一碳源时的生长速率、异养硝化和好氧反硝化性能最好,并且利用无机氮源的能力比有机氮源能力强。
[Abstract]:Compared with the traditional denitrification bacteria, heterotrophic nitrification-aerobic denitrifying bacteria have great advantages in denitrification and have attracted wide attention. A heterotrophic nitrification-aerobic denitrifying bacterium with high denitrification performance was isolated from activated sludge with acetamide as the sole nitrogen source and named Y1. After morphological observation, physiological and biochemical characteristics and 16s rRNA analysis, Acinetobaterjohnsonii was identified as Acinetobacter yoelii, and Gram staining was negative. The physiological and biochemical identification of Y1 strain showed that Y1 was positive for indole, citrate, hydrogen sulfide and contact enzyme, which indicated that Y1 could make good use of the above substances. The results of methyl red, glucose fermentation, sucrose fermentation, gelatin liquefaction, starch hydrolysis, oxidase and urease tests were negative, indicating that the strain could not make good use of the above substances. In order to test the denitrification performance of Y1 strain, it was cultured in heterotrophic nitrification medium and aerobic denitrification medium respectively. Within 108 h, the removal rate of ammonia nitrogen in heterotrophic nitrification medium inoculated with Y1 strain was about 66.9%. The removal rate was 0.53 mg.L~ (-1) h ~ (-1), the removal rate of nitrate was about 100 mg, and the removal rate was 0.10 mg L ~ (-1) h ~ (-1). Within 84 h, the removal rate of nitrate in aerobic denitrification medium inoculated with Y1 strain was 69.7 and the removal rate was 0.74 mg L ~ (-1) h ~ (-1). The above results showed that the denitrification performance of Y1 strain was higher than that of Y1 strain. In order to further study the growth requirements of the strain and keep other conditions unchanged, the strain Y1 was cultured under different carbon and nitrogen sources, respectively. The results showed that the growth rate of strain Y1 was when sodium succinate was the sole carbon source. Heterotrophic nitrification and aerobic denitrification were the best, and the ability of using inorganic nitrogen sources was better than that of organic nitrogen sources.
【作者单位】：武汉理工大学土木工程与建筑学院;武汉理工大学化学化工与生命科学学院;
【基金】：武汉理工大学国家级大学生创新创业训练计划项目(20161049706007) 国家自然科学基金项目(31400435;21407114) 湖北省自然科学基金项目(2015CFB596)
【分类号】：X172

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