接种根内球囊霉对白三叶抗铜毒害的影响

发布时间：2019-03-22 08:48

【摘要】：采用盆栽试验研究丛枝菌根(AM)真菌根内球囊霉(Glomus intraradices)接种白三叶(Trifolium repens L.)对高度铜(Cu)污染(300mg/kg)的生理生化反应。结果表明:铜污染降低了白三叶单株干重、叶片光合色素、可溶性糖、脯氨酸、过氧化氢酶(CAT)及各部位磷(P)水平,而提高了叶片相对电导率、过氧化物酶(POD)、抗坏血酸(ASA)、过氧化氢(H_2O_2)、地上部镁(Mg)和各部位Cu水平以及Cu迁移量;AM真菌显著提高了Cu污染条件下白三叶单株干重、叶片叶绿素、可溶性糖、脯氨酸、POD、CAT、ASA、P、Cu及各部位Mg水平,降低了叶片相对电导率和H_2O_2含量;在Cu正常和Cu污染条件下,接种株的Cu迁移量均显著高于未接种株。可见,接种根内球囊霉不仅促进了Cu污染条件下白三叶生长及Cu在地上部的积累量,还通过提高渗透调节和抗氧化能力来增强其抗Cu毒害能力。
[Abstract]:A pot experiment was conducted to study the inoculation of (Trifolium repens L.) with (Glomus intraradices) of arbuscular mycorrhizal (AM), a fungal fungus, in the roots of White clover. Physiological and biochemical reactions to high copper (Cu) pollution (300mg/kg). The results showed that copper pollution reduced the dry weight per plant, photosynthetic pigment, soluble sugar, proline, catalase (CAT) and phosphorus (P) levels in leaves of white clover, but increased leaf relative conductivity and peroxidase (POD),. Ascorbic acid (ASA), hydrogen peroxide (H_2O_2), above ground magnesium (Mg) and Cu levels in various parts and Cu migration; AM fungi significantly increased the dry weight of white clover leaves, chlorophyll, soluble sugar, proline, POD,CAT,ASA,P,Cu and Mg levels, and decreased the relative conductivity and H_2O_2 content of leaves under the condition of Cu pollution. Under the condition of normal Cu and Cu pollution, the Cu migration of inoculated plants was significantly higher than that of non-inoculated plants. It can be seen that inoculation not only promoted the growth of white clover and the accumulation of Cu in shoot under the condition of Cu pollution, but also enhanced the ability of anti-Cu toxicity by improving the osmotic adjustment and anti-oxidation ability.
【作者单位】：安徽师范大学生命科学学院/生物环境与生态安全安徽省重点实验室/皖江城市带退化生态系统的恢复与重建安徽省协同创新中心;
【基金】：国家级大学生创新创业训练计划项目(201510370060)
【分类号】：X173;S541.2

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