海南草豆蔻及其主要活性组分、金属元素含量的谱学测定

发布时间：2018-03-03 16:40

  本文选题：海南草豆蔻　切入点：山姜素　出处：《光谱学与光谱分析》2016年04期 　论文类型：期刊论文

【摘要】：采用红外光谱、紫外光谱、荧光光谱及微波消解电感耦合等离子体质谱法对海南白沙和霸王岭两个产地的草豆蔻进行了分析。其乙醇提取物的红外光谱都具有1 051,1 390,2 976及3 300cm~(-1)左右表征黄酮类成分的特征吸收峰,二阶红外导数谱图显示了在2 977.72及2 899.94有相似吸收峰,在1 922.36及1 650.87cm~(-1)有显著差异吸收峰,可以鉴定区分海南不同产地的草豆蔻。利用紫外光谱法表征了两个不同产地草豆蔻的特征谱图,并根据有关的吸收原理,建立了测定草豆蔻植物中主要组分山姜素与豆蔻明含量的方法,获得了良好的分析精密度(0.42%~0.29%)和回收率(84.28%~117.41%)。测得白沙草豆蔻中山姜素和豆蔻明的含量分别为4.23%和3.83%,而霸王岭的分别为3.72%和3.34%。荧光测定结果表明,不同产地的草豆蔻乙醇提取物具有相似的荧光发射及激发光谱谱图,荧光强度的差别也再次证明白沙草豆蔻乙醇提取物的浓度大于霸王岭的。利用微波消解电感耦合等离子体质谱法分析了草豆蔻中的K,Ca,Mg,Na,Al,Fe,Mn,Cu,Zn等十七种金属元素及其含量。该研究为定性、定量地鉴定区别不同产地或不同种植批次的草豆蔻及测定其中主要活性组分的含量建立了简单可行的方法;根据实验测得的金属元素种类及含量的结果,可为进一步开发利用草豆蔻及提高食品安全意识提供更好的理论依据。
[Abstract]:Infrared spectrum, ultraviolet spectrum, Fluorescence spectra and microwave digestion inductively coupled plasma-mass spectrometry (ICP-MS) were used to analyze cardamom from Baisha and Bawangling, Hainan Province. The infrared spectra of ethanol extracts were characterized by about 1 051C 1 390C 2976 and 3 300 cm ~ (-1) cm ~ (-1) of flavonoids. Characteristic absorption peaks of similar components, The second order infrared derivative spectra showed that there were similar absorption peaks at 2977.72 and 2899.94, and significant differential absorption peaks at 1 922.36 and 1 650.87 cm ~ (-1). It is possible to identify and distinguish the cardamom from different habitats in Hainan. The characteristic spectra of two different areas of cardamom were characterized by ultraviolet spectroscopy, and according to the principle of absorption, A method for the determination of curcumin and cardamine in cardamom was established. A good analytical precision of 0.42 and a recovery rate of 84.28 and 117.41 were obtained. The contents of curcumin and cardamine were 4.23% and 3.83, respectively, and those of Bawangling were 3.72% and 3.34, respectively. The ethanol extracts from different producing areas have similar fluorescence emission and excitation spectra. The difference of fluorescence intensity also proves again that the concentration of ethanol extract of Cortex cardamonis is higher than that of Bawangling. 17 kinds of metal elements and their contents in Caommeg cardamom are analyzed by microwave digestion inductively coupled plasma-mass spectrometry (ICP-MS). The study was qualitative, A simple and feasible method was established to quantitatively identify and determine the contents of main active components of cardamom from different producing areas and different planting lots, and to establish a simple and feasible method for the determination of metal elements and their contents according to the experimental results. It can provide a better theoretical basis for the further development and utilization of cardamom and food safety awareness.
【作者单位】： 海南师范大学化学与化工学院;海南师范大学地理与旅游学院;海南省热带药用植物化学重点实验室;
【基金】：国家自然科学基金项目(31360069) 海南省中药现代化项目(ZY201330)资助
【分类号】：O657;R284.1
，

本文编号：1561840