电喷雾离子迁移谱检测氨基酸及多肽的研究

发布时间：2018-05-02 22:01

本文选题：电喷雾 + 离子迁移谱　；参考：《分析化学》2017年11期

【摘要】：建立了氨基酸及多肽的电喷雾离子迁移谱检测方法。采用自制的电喷雾离子迁移谱装置,在室温条件下以甲醇为溶剂,空气为漂移气体,流速为1000 m L/min,电喷液流速为2!L/min,测试了甘氨酸、胱氨酸、组氨酸、精氨酸4种氨基酸及缓激肽片段(1~7)和P物质2种多肽的离子迁移谱,计算出上述化合物的约化迁移率。离子迁移谱图反映出化合物的结构信息,具有指纹谱特征。此装置在1 min的检测时间内对P物质的检测灵敏度达到855 ng/m L。结果表明,电喷雾离子迁移谱可用于氨基酸及多肽类化合物的现场快速鉴定。
[Abstract]:A method for the determination of amino acids and polypeptides by electrospray ion migration spectroscopy was established. A self-made electrospray ion-transfer spectrometer was used to measure glycine, cystine and histidine at room temperature with methanol as solvent, air as drift gas, flow rate of 1000 mL / min and flow rate of 2 L / min. The ion-transport spectra of arginine 4 amino acids and bradykinin fragments 1 ~ 7) and substance P were used to calculate the reduced mobility of these compounds. The ion transport spectra reflect the structure information of the compounds and have the characteristic of fingerprint spectrum. The sensitivity of the device to the detection of substance P within 1 min was 855 ng/m / L. The results showed that the electrospray ion transport spectra could be used to identify amino acids and polypeptides in situ.
【作者单位】：防化研究院;中国科学院大连化学物理研究所;
【分类号】：O629.7;O657

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