基于UPLC及一测多评法同时测定苁蓉总苷胶囊中的活性成分

发布时间：2018-06-17 02:06

本文选题：一测多评 + 苁蓉总苷胶囊　；参考：《中国实验方剂学杂志》2017年10期

【摘要】：目的:采用"一测多评"(QAMS)技术建立超高效液相法(UPLC)同时测定苁蓉总苷胶囊中6种成分的分析方法,并与标准曲线法进行比较,评价一测多评法用于苁蓉总苷胶囊质量控制的技术适应性和应用可行性。方法:以苁蓉总苷胶囊为研究对象,有机酸类成分以绿原酸为内参物,建立其与咖啡酸的相对校正因子;苯乙醇苷类成分以毛蕊花糖苷为内参物,建立其与管花苷A,异毛蕊花糖苷,松果菊苷间的相对校正因子,同时考察了各相对校正因子的耐用性,并比较该方法与标准曲线法测定结果的差异,以验证一测多评法的准确性和可行性。结果:有机酚酸类成分绿原酸与咖啡酸的相对校正因子为0.538,苯乙醇苷类成分毛蕊花糖苷与管花苷A,异毛蕊花糖苷,松果菊苷间的相对校正因子分别为1.346,1.266和1.135,各相对校正因子的耐用性较好,10批样品QAMS法的计算结果与标准曲线法的测定结果无显著性差异,实验所得的相对校正因子可信。结论:QAMS法可以作为一种简便可靠的质量评价模式用于苁蓉总苷胶囊多种成分的定量测定方法。
[Abstract]:Objective: to establish a method for the simultaneous determination of six components in Cistanche glycoside capsules by using "one test and multiple evaluation" QAMS technique, and to compare it with the standard curve method. To evaluate the technical adaptability and application feasibility of one-test multi-evaluation method for quality control of Cistanche glycoside capsules. Methods: taking Cistanche glycoside capsule as the research object, the organic acids were used as the internal reference material, and the relative calibration factor between the organic acids and caffeic acid was established, and the phenylethanoside was used as the internal reference material, and the phenylethanoside was used as the internal reference. The relative correction factors were established and the durability of the relative correction factors were investigated, and the difference between the method and the standard curve method was compared. In order to verify the accuracy and feasibility of a multi-evaluation method. Results: the relative correction factor of Lv Yuan acid and caffeic acid was 0.538. The relative correction factors were 1.346 and 1.135, respectively. The results of QAMS method for 10 batches of samples had no significant difference with that of the standard curve method. The relative correction factors obtained from the experiments were credible. Conclusion this method can be used as a simple and reliable method for the quantitative determination of various components in Cistanche glycoside capsules.
【作者单位】：南京中医药大学;江苏康缘药业股份有限公司;中药制药过程新技术国家重点实验室;
【基金】：国家“重大新药创制”科技重大专项(2013ZX09402203)
【分类号】：R286.0;O657.7

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