HPLC法同时测定大黄-黄连药材中5种蒽醌类成分的含量及最优配伍研究
发布时间：2018-09-03 14:39
【摘要】：目的:建立同时测定大黄-黄连药材中5种蒽醌类成分含量的方法,并优选大黄-黄连药材质量比。方法:采用高效液相色谱法。色谱柱为Zorbax SB-C_(18)(250 mm×4.6 mm,5μm),流动相为乙腈-0.05 mol/L磷酸二氢钾(48:52,V/V,每100 mL中加十二烷基硫酸钠0.4 g,磷酸调节p H至4.0),流速为1 mL/min,检测波长为254 nm,柱温为25℃,进样量为10μL。考察大黄-黄连药材质量比为1:1、1:2、2:1、2:3、3:2时配伍药材中芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚的含量。结果:芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚进样量分别在0.652~3.081、0.704~3.422、1.280~6.197、0.633~0.324、1.326~5.954μg范围内与各自峰面积积分值呈良好线性关系(r≥0.999 7),精密度、稳定性、重复性试验的RSD均小于2.0%(n=6),平均加样回收率为98.92%~100.37%(RSD≤2.26%,n=6)。大黄-黄连药材质量比为2:1时,5种蒽醌类成分总量最高。结论:本方法精密度、稳定性、重复性良好,可用于不同配伍比大黄-黄连药材的质量检定;大黄-黄连药材的最佳质量比为2:1。
[Abstract]:Objective: to establish a method for the simultaneous determination of five anthraquinones in rhubarb and Coptis chinensis, and to optimize the mass ratio of rhubarb and Coptis chinensis. Methods: high performance liquid chromatography was used. The chromatographic column was Zorbax SB-C_ (18) (250 mm 脳 4.6 mm,5 渭 m), mobile phase was acetonitrile-0.05 mol/L potassium dihydrogen phosphate (48: 52 V / V, 0.4 g sodium dodecyl sulfate per 100 mL, adjusted pH to 4.0 by phosphoric acid). The flow rate of 1 mL/min, was 25 鈩，
本文编号：2220266
[Abstract]:Objective: to establish a method for the simultaneous determination of five anthraquinones in rhubarb and Coptis chinensis, and to optimize the mass ratio of rhubarb and Coptis chinensis. Methods: high performance liquid chromatography was used. The chromatographic column was Zorbax SB-C_ (18) (250 mm 脳 4.6 mm,5 渭 m), mobile phase was acetonitrile-0.05 mol/L potassium dihydrogen phosphate (48: 52 V / V, 0.4 g sodium dodecyl sulfate per 100 mL, adjusted pH to 4.0 by phosphoric acid). The flow rate of 1 mL/min, was 25 鈩，
本文编号：2220266
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