基于UPLC-Q-TOF-MS技术的广地龙化学成分分析
发布时间：2018-10-08 07:55
【摘要】：目的建立广地龙药材中化学成分的超高液相色谱-质谱联用(UPLC-Q-TOF-MS)分析方法。方法采用Waters ACQUITY UPLC BEH C18(100 mm×2.1 mm,1.7μm)色谱柱,以0.1%甲酸水-0.1%甲酸乙腈为流动相梯度洗脱,体积流量0.3 m L/min,柱温35℃,正负离子模式扫描。结果共鉴定出广地龙药材中84个化学成分,包括11种游离氨基酸、26种有机酸类、11种核苷类、5种二肽及环二肽类、21种含氮类物质和10种其他类。结论该法准确、快速、灵敏,为进一步阐明其药效物质基础和后期质量控制指标的选择提供科学依据。
[Abstract]:Objective to establish a method for the determination of chemical constituents in Rhizoma Guandelanensis by ultra high liquid chromatography-mass spectrometry (UPLC-Q-TOF-MS). Methods Waters ACQUITY UPLC BEH C18 (100 mm 脳 2.1 mm,1.7 渭 m) column was used, with 0.1% formic acid water and 0.1% formic acid acetonitrile as mobile phase gradient elution, volume flow rate 0.3 mL / min, column temperature 35 鈩，
本文编号：2256004
[Abstract]:Objective to establish a method for the determination of chemical constituents in Rhizoma Guandelanensis by ultra high liquid chromatography-mass spectrometry (UPLC-Q-TOF-MS). Methods Waters ACQUITY UPLC BEH C18 (100 mm 脳 2.1 mm,1.7 渭 m) column was used, with 0.1% formic acid water and 0.1% formic acid acetonitrile as mobile phase gradient elution, volume flow rate 0.3 mL / min, column temperature 35 鈩，
本文编号：2256004
本文链接：https://www.wllwen.com/kejilunwen/huaxue/2256004.html
教材专著
