钠钾替代条件下不同基因型棉花叶片的FTIR光谱研究

发布时间：2018-10-08 08:06

【摘要】：钾缺乏是制约棉花生长的重要因子之一,而钠钾替代的研究一直是国内外关注的重点。以不同钾效率基因型棉花(钾高效HG103和钾低效LG122)为材料,利用傅里叶红外光谱(FTIR)对不同钾钠处理下棉花叶片的物质组成进行研究,分析不同钾钠条件下,叶片红外光谱图谱的差异,探讨钠钾替代对其物质成分和结构的影响,以及基因型的差异。结果表明:(1)缺钾条件下施钠,2 960,2 855,2 926,1 103和1 078cm~(-1)这5个特征峰的相对吸光度均有所升高,其中HG103在2 960,2 855和2 926cm~(-1)处增加的量与LG122几乎一致,而在1 103和1 078cm~(-1)附近,HG103增加的量高于LG122;然而适钾的条件下施钠,这5个特征峰的相对吸光度都有所减少,且HG103减少的量都小于LG122,表明缺钾时施钠促进糖类、蛋白质和酯类的合成,其中对糖类的促进作用是HG103的大于LG122的,而对蛋白质和酯类的则没有差异;适钾时施钠则有抑制作用,对HG103的抑制作用小于LG122。(2)缺钾时,1 734和1 437cm~(-1)附近的特征峰缺失,而补充钠后这些缺失的峰也未出现,说明缺钾破坏了叶片中蛋白质酰胺Ⅰ带和纤维素的结构,而缺钾补充钠并不能缓解这种破坏,即对于钾的这些功能,钠不能完全代替钾。
[Abstract]:Potassium deficiency is one of the important factors restricting cotton growth, and the study of sodium and potassium substitution has been the focus of attention at home and abroad. Using different potassium efficiency genotypes of cotton (potassium efficient HG103 and potassium low efficient LG122) as materials, Fourier transform infrared spectroscopy (FTIR) was used to study the composition of cotton leaves under different potassium and sodium treatments. The effects of sodium and potassium substitution on the composition and structure of leaves and the differences of genotypes were discussed. The results showed that: (1) the relative absorbance of the five characteristic peaks of Na ~ (2 960N) 2 985 ~ (2 926) ~ (1 103) and 1 078 cm ~ (-1) increased under the condition of potassium deficiency, and the amount of increase of HG103 at 2 960 ~ (2 855) and 2 926 cm ~ (-1) was almost the same as that of LG122. However, the increase of HG103 in the vicinity of 1 103 and 1 078 cm ~ (-1) was higher than that of LG122;, however, the relative absorbance of the five characteristic peaks decreased, and the decrease of HG103 was lower than that of LG122,. In the synthesis of proteins and esters, the promoting effect of carbohydrate on HG103 is greater than that of LG122, but there is no difference between protein and ester. The inhibitory effect of HG103 on HG103 was less than that on LG122. (2) at K deficiency (1 734 and 1 437 cm ~ (-1), but these missing peaks did not appear after sodium supplementation, which indicated that potassium deficiency destroyed the structure of protein amide I band and cellulose in leaves. Potassium deficiency does not alleviate this damage, that is, sodium cannot completely replace potassium for these functions.
【作者单位】：华中农业大学资源与环境学院农业部长江中下游耕地保育重点实验室;
【基金】：国家自然科学基金项目(40801112) 公益性行业(农业)科研专项项目(201203013)资助
【分类号】：S562;O657.33

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