铜绿假单胞菌存活时间延长可提高生物燃料电池的产电量

发布时间：2018-05-06 12:30

本文选题：铜绿假单胞菌 + 微生物燃料电池　；参考：《生物工程学报》2017年04期

【摘要】：铜绿假单胞菌产生的次生代谢产物吩嗪化合物具有电子传递作用,可用于构建微生物燃料电池。如何通过改进微生物自身性质来提升微生物燃料电池产电量是研究的热点与难点之一。本文以铜绿假单胞菌SJTD-1和其敲除突变株SJTD-1(ΔmvaT)为对象,研究了以其搭建的微生物燃料电池的放电过程,分析了影响其放电量的主要因素。结果显示,假单胞菌产生的吩嗪化合物和发酵系统中细菌的活性与存活数量均会直接影响燃料电池的产电量。敲除突变株SJTD-1(ΔmvaT)可产生较多的吩嗪化合物,在生物燃料电池系统可持续放电超过160 h,产生2.32 J的总电量;而野生菌株SJTD-1仅能放电90 h,产生1.30 J的总电量。细胞生长分析结果进一步显示,与野生菌株相比,突变菌株SJTD-1(ΔmvaT)在发酵过程中维持了较长的稳定期生长,细胞存活时间更长,放电时间更持久。因此,铜绿假单胞菌存活时间延长,可增加其在微生物燃料电池中的放电时间,从而提升微生物燃料电池的总产电量。本研究可为通过工程菌株改造来提升微生物燃料电池总产电量的研究提供思路,有利于推进微生物燃料电池的实际应用。
[Abstract]:Phenazine compounds, a secondary metabolite produced by Pseudomonas aeruginosa, can be used for the construction of microbial fuel cells. How to improve the properties of microbial fuel cells is one of the hot and difficult research topics. In this paper, the discharge process of Pseudomonas aeruginosa (SJTD-1) and its knockout mutant SJTD-1 (螖 MvaT-1) were studied and the main factors affecting the discharge were analyzed. The results showed that the phenazine compounds produced by Pseudomonas sp. And the activity and survival quantity of bacteria in the fermentation system could directly affect the energy production of fuel cells. Knockout mutant SJTD-1 (螖 MvaT) can produce more phenazine compounds, which can discharge continuously for more than 160h in the biofuel cell system, producing 2.32J total electric energy, while the wild strain SJTD-1 can only discharge for 90 h, producing 1.30J total electric energy. The results of cell growth analysis further showed that the mutant strain SJTD-1 (螖 MvaT) maintained a longer stable growth period, longer cell survival time and longer discharge time than wild strain. Therefore, the prolonged survival time of Pseudomonas aeruginosa can increase its discharge time in microbial fuel cells, thus increasing the total power production of microbial fuel cells. This study can provide some ideas for improving the total energy production of microbial fuel cells through the transformation of engineering strains, and is helpful to promote the practical application of microbial fuel cells.
【作者单位】：上海交通大学生命科学技术学院微生物代谢国家重点实验室;上海工程技术大学材料工程学院;
【基金】：国家自然科学基金(Nos.31370152,31570099) 上海市浦江人才计划(No.14PJD020)资助~~
【分类号】：TM911.45

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