光周期和温度对生物钟基因cwo在棉铃虫幼虫节律表达的影响

发布时间：2017-12-26 20:16

本文关键词：光周期和温度对生物钟基因cwo在棉铃虫幼虫节律表达的影响　出处：《应用昆虫学报》2016年05期 　论文类型：期刊论文

【摘要】：【目的】生物钟普遍存在于生物体的生长发育、行为及生理等各个方面。本研究旨在探究光周期和温度对生物钟基因cwo在棉铃虫Helicoverpa armigera幼虫昼夜节律表达的影响。【方法】通过转录组测序获得了生物钟基因cwo的c DNA开放阅读框全长,利用实时荧光定量PCR技术分析了生物钟基因cwo在棉铃虫幼虫中的时空表达,以及其在不同光周期和不同温度条件下的昼夜表达节律。【结果】序列分析结果显示,棉铃虫生物钟基因cwo的c DNA序列开放阅读框为1 335 bp,编码444个氨基酸残基,预测蛋白的分子质量为49.95 ku。时空表达分析表明,cwo基因在棉铃虫幼虫组织中以脑和腹神经索表达量较高,在卵孵化期、蜕皮期、蛹变态期及羽化前1 d高峰度表达。头部昼夜节律表达分析表明,正常光周期条件下生物钟基因cwo呈昼夜节律表达,在暗期高表达;短时间持续暗期/光期处理使cwo表达的时间相位提前,长时间持续暗期/光期处理使cwo表达的节律性显著降低;38℃处理使cwo表达短期内显著上调,并改变了其节律性,18℃处理使cwo表达在暗期显著下调,但是节律性不变。【结论】生物钟基因cwo在棉铃虫幼虫头部的昼夜节律表达受到光周期和温度的影响。
[Abstract]:[Objective] biological clocks are common in the growth, development, behavior and physiology of organisms. The aim of this study was to investigate the effect of photoperiod and temperature on the circadian rhythm expression of the clock gene CWO in the Helicoverpa armigera larvae of Helicoverpa armigera. [method] through transcriptome sequencing the clock gene CWO C DNA ORF, analyzed the biological clock gene CWO in cotton bollworm larvae in the temporal and spatial expression using real-time fluorescence quantitative PCR technology, and the different photoperiod and temperature condition of circadian rhythm. [result] sequence analysis showed that the C DNA sequence of Helicoverpa armigera CWO was 1335 BP, encoding 444 amino acid residues, and the predicted protein molecular weight was 49.95 Ku. The temporal and spatial expression analysis showed that the expression of CWO gene in the larvae and tissues of Helicoverpa armigera was higher in the brain and ventral cord than in the hatching stage, the molting stage, the pupal metamorphosis stage and the 1 D peak stage before emergence. Expression analyses showed the head of circadian rhythm, normal photoperiod conditions showed circadian clock gene CWO expression and high expression in the dark period; short duration of dark phase of the light / time of expression of CWO in the early stage, long time continuous dark period / rhythmic light treatment of the expression of CWO was significantly lower at 38; the expression of CWO increased significantly in the short term, and changed the rhythm of the 18 treatment that CWO is downregulated in the dark period, but the rhythm of the same. [Conclusion] the circadian rhythm expression of the clock gene CWO in the head of Helicoverpa armigera is influenced by the photoperiod and temperature.
【作者单位】：中国农业大学昆虫系;新疆博尔塔拉蒙古自治州农业技术推广中心;
【基金】：国家科技支撑计划2012BAD19B05
【分类号】：S433
【正文快照】： 地球表面光照和温度随着地球自转和公转不断进行着周期性变化,产生了昼夜和季节的交替,生物体为了适应这种外界环境,经过长期进化在生理、形态、行为等生命活动中表现出了不同的生物节律。生物节律由生物钟控制,生物钟通常指生物体内激发生物节律并使之维持稳定的内部定时机制

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