转磷酸甘露糖变位酶基因提高水稻维生素C含量

发布时间：2017-12-28 05:23

本文关键词：转磷酸甘露糖变位酶基因提高水稻维生素C含量　出处：《中国水稻科学》2016年04期 　论文类型：期刊论文

【摘要】：维生素C(VC)是人体健康所必需的营养元素。人类由于缺乏VC合成途径中的最后一种酶(L-古洛糖酸内酯氧化酶),自身不能合成VC。水稻是重要的粮食作物,增加水稻种子中VC含量,能够提高其营养价值。磷酸甘露糖变位酶(PMM)是VC合成通路中一种重要的酶,催化甘露糖-6-磷酸到甘露糖-1-磷酸的转变。将水稻PMM基因(OsPMM)构建在双右边界双元载体pMNDRBBin6上,并用种子特异表达的启动子BX14驱动其表达。通过农杆菌介导的转化系统,OsPMM基因被转入粳型三系恢复系C418中。通过分子检测,在T_2代筛选到了无选择标记的转基因植株。对OsPMM基因在转基因植株中的表达进行分析,发现OsPMM基因在转基因水稻种子内的表达水平明显提高,相应地,转基因系种子中的VC含量也提高了25%~50%。
[Abstract]:Vitamin C (VC) is essential for the health of the human body. Due to the lack of the last enzyme (L- palacate oxidase) in the VC synthesis pathway, human beings can not synthesize VC. Rice is an important grain crop. Increasing the content of VC in rice seeds can improve the nutritional value of rice. The phosphoric mannose mutant enzyme (PMM) is an important enzyme in the VC synthesis pathway, which catalyzes the transformation of mannose -6- phosphoric acid to mannose -1- phosphoric acid. The rice PMM gene (OsPMM) was constructed on double right dual vector pMNDRBBin6, and the expression was driven by seed specific promoter BX14. Through agrobacterium mediated transformation system, the OsPMM gene was transferred into the japonica type three line recovery line C418. Through molecular detection, transgenic plants with no selectable markers were screened in the T_2 generation. The expression of OsPMM gene in transgenic plants was analyzed. It was found that the expression level of OsPMM gene in transgenic rice seeds increased significantly. Correspondingly, the VC content in transgenic lines increased by 25%~50%.
【作者单位】：江汉大学系统生物学研究院;中国科学院遗传与发育生物学研究所;海南大学生命科学研究院;
【基金】：国家转基因生物新品种培育重大专项(2014ZX08001-002) 国家自然科学基金资助项目(31300999) 湖北省教育厅项目(B2015229) 武汉市科学技术局项目(2014072704011250) 江汉大学科研启动项目(3003-06000043)
【分类号】：S511
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