广泛耐药肺炎克雷伯菌生物膜形成能力及毒力基因相关分析

发布时间：2018-01-02 19:07

本文关键词：广泛耐药肺炎克雷伯菌生物膜形成能力及毒力基因相关分析　出处：《临床检验杂志》2016年06期 　论文类型：期刊论文

【摘要】：目的了解广泛耐药(extensively drug-resistant,XDR)肺炎克雷伯菌生物膜形成能力及毒力基因分布特征。方法收集温州医科大学附属第一医院2013年5月至7月暴发流行的XDR肺炎克雷伯菌15株和同时期分离的敏感肺炎克雷伯菌30株。采用结晶紫染色法检测肺炎克雷伯菌体外生物膜形成能力,PCR方法筛查菌株毒力相关基因携带情况,并比较分析2组菌株生物膜形成能力和毒力基因的分布情况。结果 XDR肺炎克雷伯菌株与敏感菌株形成生物膜A590 nm值分别为0.505 3±0.291 2和0.429 8±0.235 6,二者差异无统计学意义(t=0.87,P=0.38)。15株XDR肺炎克雷伯菌中未检出荚膜多糖基因,而敏感菌株中荚膜多糖基因wzy-K2、wzy-K3和wzy-K57的检出率分别为10.0%、3.3%和6.7%。其他毒力相关基因ure A、wab G、mrk D、fim H和uge在XDR耐药菌株和敏感菌株的检出率均为100.0%,除上述5种毒力基因外,仅1株XDR肺炎克雷伯菌检出kfu BC毒力基因(6.7%)。而敏感菌株中kfu BC、rmp A、wca G、all S、iuc B、iro NB和mag A中检出率分别为20.0%、20.0%、10.0%、16.7%、40.0%、10.0%和10.0%,毒力基因iuc B检出率与XDR菌株相比,差异具有统计学意义(χ2=6.264,P=0.012)。结论 XDR肺炎克雷伯菌的生物膜形成能力与敏感菌株间无明显差异,其毒力谱较为单一,携带的毒力因子种类亦较敏感菌株少。
[Abstract]:Objective to investigate the extensive drug resistant drug-resistant. XDR). Methods 15 strains of Klebsiella pneumoniae were collected from the first affiliated Hospital of Wenzhou Medical University from May 2013 to July and at the same time. Thirty strains of Klebsiella pneumoniae were isolated. The biofilm forming ability of Klebsiella pneumoniae in vitro was detected by crystal violet staining. PCR method was used to screen virulence related genes. The biofilm forming ability and the distribution of virulence genes in two groups of strains were compared and analyzed. Results the A590nm value of biofilm formation of Klebsiella pneumoniae strain and sensitive strain was 0.505, respectively. 3 卤0.291 2 and 0.429 8 卤0.235 6. There was no significant difference between the two strains. No capsule polysaccharide gene was detected in XDR Klebsiella pneumoniae, but the capsule polysaccharide gene wzy-K2 was found in sensitive strain of Klebsiella pneumoniae. The detectable rates of wzy-K3 and wzy-K57 were 10.0% and 6.7%, respectively. Other virulence related genes ure AWAb Gnmrk D. The detectable rates of fim H and uge in both XDR resistant and sensitive strains were 100.0, except for the five virulence genes mentioned above. Only one strain of Klebsiella pneumoniae XDR detected kfu BC virulence gene 6.70.The sensitive strain was kfu BCrmp Awca Gnall siuc B. The detectable rate of iro NB and mag A were 20.0 and 10.0, respectively. The detection rate of virulence gene iuc B was significantly different from that of XDR strain (蠂 2 + 6.264). Conclusion the biofilm forming ability of Klebsiella pneumoniae is not significantly different from that of sensitive strains, and its virulence spectrum is single, and the types of virulence factors carried by Klebsiella pneumoniae are less than those of sensitive strains.
【作者单位】：温州医科大学附属第一医院医学检验中心;浙江医院检验科;温州医科大学检验医学院生命科学学院;
【基金】：国家自然科学基金(81171614) 浙江省卫生高层次创新人才培养工程(浙卫发[2012]241号)
【分类号】：R440
【正文快照】： 肺炎克雷伯菌是临床常见院内感染致病菌,可引起肺炎、肝脓肿、血流感染等,具有较高的致病性[1]。随着抗菌药物的滥用及侵入性治疗措施的广泛使用,广泛耐药(extensively drug-resistant,XDR)肺炎克雷伯菌的检出率日益增加,给临床治疗带来严重威胁。有研究认为,耐药菌株与敏感菌

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