肝癌中TLR3信号分子与自噬相关基因表达的相关性及临床意义

发布时间：2018-01-03 09:58

本文关键词：肝癌中TLR3信号分子与自噬相关基因表达的相关性及临床意义　出处：《南京医科大学学报(自然科学版)》2016年11期 　论文类型：期刊论文

【摘要】：目的 :研究肝细胞肝癌(hepatocellular carcinoma,HCC)中Toll样受体3(Toll-like receptor 3,TLR3)及信号分子含TIR结构域的转接蛋白(TIR-domain-containing adaptor protein including IFN-β,TRIF)表达与细胞自噬相关蛋白Beclin1和LC3表达的相关性及临床意义,并探讨相关机制。方法:收集与随访101例HCC病例,构建肝组织芯片。HE染色判断HCC的组织分化,免疫组化染色检测HCC组织中Ki-67、TLR3、TRIF、LC3和Beclin l的表达,应用TUNEL方法检测肿瘤细胞凋亡信号。统计学分析其临床病理因素和预后。结果:本组HCC中TLR3和TRIF表达呈正相关(ρ=0.640,P0.001),其高表达分别与肿瘤Hbs Ag感染、硬化背景、细胞凋亡指数(apoptosis index,AI)及患者5年总生存率呈正相关,与肿瘤细胞增殖、血管浸润、Edmondson分级和TNM分期呈负相关。Beclin1和LC3表达呈显著正相关(ρ=0.587,P0.001),两者高表达分别与肿瘤脉管浸润、Edmondson分级、TNM分期、肿瘤细胞增殖呈正相关,与AI和预后呈负相关。并且TLR3、TRIF分别与LC3和Beclin1的表达呈负相关(P均0.001)。结论:HCC中TLR3及其信号分子TRIF表达,细胞自噬相关基因Beclin1和LC3的表达分别通过调控细胞增殖、凋亡和自噬来影响肿瘤的生物学行为和预后。HCC组织中Beclin1介导的自噬途径与TLR3信号凋亡通路存在交叉,两条通路异常可能是HCC发生、进展的机制之一。
[Abstract]:Objective: To study the hepatocellular carcinoma (hepatocellular, carcinoma, HCC) Toll like receptor 3 (Toll-like receptor 3, TLR3) and signaling adaptor protein containing TIR domain (TIR-domain-containing adaptor protein including IFN- beta, TRIF) expression and autophagy related protein expression of Beclin1 and LC3 and clinical significance, and to explore the related mechanism methods: the collection and follow-up of 101 cases of HCC, construct the liver tissue chip.HE staining to determine the differentiation of tissue HCC, immunohistochemical staining of Ki-67, detection of HCC tissue TLR3, TRIF, LC3 and Beclin L expression and apoptosis of tumor cells by TUNEL signal detection method. To analyze the clinicopathological factors and prognosis of statistical results. TLR3 and TRIF: the expression of HCC was positively correlated (=0.640, P0.001), the high expression of Hbs and Ag were infection, tumor sclerosis background cell apoptosis index (apoptosis, index, AI) and in patients with a total of 5 years The survival rate was positively correlated with tumor cell proliferation, vascular invasion, Edmondson stage and TNM stage was negatively correlated with.Beclin1 and LC3 expression were positively correlated (=0.587, P0.001), the high expression of both Edmondson and vascular infiltration, tumor grade, TNM stage, tumor cell proliferation was positively correlated with AI and a negative correlation with prognosis and TLR3, TRIF were negatively correlated with the expression of LC3 and Beclin1 respectively (P < 0.001). Conclusion: the expression of TLR3 and HCC in TRIF signaling molecules, the expression of autophagy related genes Beclin1 and LC3 respectively through the regulation of cell proliferation, autophagy pathway and TLR3 signal pathway of apoptosis of tumor biological behavior and prognosis of.HCC in Beclin1 the existence of cross effect mediated apoptosis and autophagy, two pathways may be abnormal HCC, one of the progress of the mechanism.

【作者单位】：南通大学医学院病理学系;
【基金】：江苏省产学研前瞻性联合研究项目(BY2013042-06) 南通市社会发展课题(HS2014001) 南通大学课题及研究生科技创新工程项目(ykc15050) 江苏省高校优势学科建设工程(PAPD)资助(苏政办发[2011]6号)
【分类号】：R735.7
【正文快照】： and clinical significance of TLR3 sig 南京医在溶酶体中发挥作 LC3、Beclin1表达与内死亡27.7%(28/101),3~ B TRIF表达的病例 ,2010,70(2): 原发性肝细胞肝癌(hepatocellular carcinoma,HCC)是人类最常见的恶性肿瘤之一,其恶性程度高,发展迅速,治疗困难,病死率

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