倭蜂猴细胞色素b基因和D-loop序列多态性分析

发布时间：2018-01-07 22:26

本文关键词：倭蜂猴细胞色素b基因和D-loop序列多态性分析　出处：《生物学杂志》2016年06期 　论文类型：期刊论文

【摘要】：对云南大围山保护区7例倭蜂猴线粒体DNA细胞色素b基因(Cytb)和控制区序列(D-loop)进行了PCR扩增、序列测定、核苷酸组成和多态性分析。结果表明:Cytb中,A、T、C和G 4种核苷酸的比例分别为29.3%、28.2%、29.8%和12.7%,A+T含量为57.5%,G+C含量为42.5%。在长度为1078 bp的Cytb序列中,存在4个变异位点,核苷酸多样性指数(π值)为0.00106,仅出现3种单倍型。7个倭蜂猴个体的遗传距离为0.000~0.003。D-loop区序列中,A、T、C和G 4种核苷酸的比例分别为31.8%、29.1%、25.0%和14.1%,A+T含量为60.9%,G+C含量为39.1%。在381 bp长的D-loop区序列中,仅存在4个变异位点,核苷酸多样性指数(π值)为0.003 00,仅出现4种单倍型。7个倭蜂猴个体的遗传距离为0.000~0.008。研究表明倭蜂猴种内个体间差异小,群体多态程度低。通过与其他物种相比,进行遗传多样性分析,倭蜂猴遗传多态性低,种群小,发生遗传漂变强度大,因此,倭蜂猴在遗传多样性水平上是迫切需要保护的。
[Abstract]:The mitochondrial DNA cytochrome b gene (Cytb) and the control region (D-loop) were amplified by PCR and sequenced. The results of nucleotide composition and polymorphism analysis showed that the proportion of four kinds of nucleotides in the two groups were 29.328.2% and 12.7%, respectively. The content of A T was 57.5% and the content of G C was 42.5. There were four mutation sites in the Cytb sequence of 1078bp. The nucleotide diversity index (蟺 value) was 0.00106, only 3 haplotypes were found. The genetic distance of 7 individuals was 0.000 ~ 0.003.D-loop sequence. The ratios of C and G were 31.8and 29.1cm 25.0% and 14.1%, respectively, and the content of A T was 60.9%. In the 381bp D-loop region, there were only four mutation sites, and the nucleotide diversity index (蟺) was 0.003 00. There were only 4 haplotypes. The genetic distance of 7 individuals was 0.000 ~ 0.008. The results showed that there was little difference among individuals and low population polymorphism. Compared with other species, the genetic distance of 7 individuals was 0.000 ~ 0.008. In the analysis of genetic diversity, the genetic polymorphism of bonobos was low, the population was small, and the intensity of genetic drift was high. Therefore, it was urgent to protect the genetic diversity of bonobos in the level of genetic diversity.
【作者单位】：云南省高校西南山地生态系统动植物生态适应进化及保护重点实验室云南师范大学生命科学学院;昆明市海口林场;
【基金】：国家自然科学基金项目(No.31260097;31560126)
【分类号】：Q953
【正文快照】： 线粒体DNA(mitochondria DNA,mt DNA)是一类核外的遗传物质,可独立进行复制与转录,相比与核DNA,它具有以下特点:母系遗传、分子结构相对简单、进化速度较快、碱基变异较大等等[1-2]。其通常用于动物遗传变异的研究,在哺乳动物中已有许多报道[3-5]。mt DNA中的细胞色素b基因(cy

【相似文献】