谷子转录因子SiNF-YA5通过ABA非依赖途径提高转基因拟南芥耐盐性

发布时间：2018-01-09 22:04

本文关键词：谷子转录因子SiNF-YA5通过ABA非依赖途径提高转基因拟南芥耐盐性　出处：《作物学报》2016年12期 　论文类型：期刊论文

【摘要】：核转录因子Y(nuclear transcription factor Y,NF-Y)类转录因子在植物生长发育和非生物胁迫响应基因表达调控中发挥重要的作用,NF-Y由3种亚基(NF-YA、NF-YB、NF-YC)组成。本研究从抗逆性强的谷子品种龙谷25中克隆1个新的NF-Y类转录因子基因Si NF-YA5。该基因序列为924 bp,编码307个氨基酸,分子量为33.76 k D,等电点为9.19。Si NF-YA5在149~210位氨基酸之间含有CBF保守结构域。亚细胞定位分析表明,Si NF-YA5定位于细胞膜和细胞核。基因功能分析显示,在不同浓度高盐处理下,和野生型拟南芥(WT)相比Si NF-YA5转基因拟南芥种子萌发率更高;苗期Si NF-YA5转基因拟南芥根表面积和植株鲜重显著高于WT,证明过表达Si NF-YA5基因可以显著提高植物耐盐性。基因表达分析结果显示,在Si NF-YA5转基因拟南芥中参与盐胁迫响应的基因NHX1和LEA7的表达量明显高于WT。另一方面,Si NF-YA5转基因拟南芥与WT相比对于ABA的敏感性差异不显著,以上结果证明Si NF-YA5主要通过ABA非依赖途径提高转基因植物对高盐胁迫的耐性。
[Abstract]:Nuclear transcription factor Y (nuclear transcription factor Y NF-Y) transcription factor growth and abiotic stress responsive gene expression in plants play an important role in the regulation of NF-Y, composed of 3 subunits (NF-YA, NF-YB, NF-YC). This study from the strong resistance of millet varieties in the Dragon Valley 25 gene cloning sequence 1 new NF-Y transcription factor gene Si NF-YA5. was 924 BP, encoding 307 amino acids, the molecular weight of 33.76 K D, isoelectric point of 9.19.Si NF-YA5 between 149~210 amino acids containing CBF conserved domain. Subcellular localization analysis showed that Si NF-YA5 was localized in the cell membrane and the nucleus. The gene function analysis showed that in different concentrations of high salt treatments, and wild type Arabidopsis (WT) compared with the higher germination rate of Si NF-YA5 transgenic seeds; seedling Si NF-YA5 transgenic Arabidopsis root surface area and plant fresh weight was significantly higher than that of WT, showed that overexpression of Si NF-Y A5 gene can significantly improve the salt tolerance of plants. Gene expression analysis showed that Si in NF-YA5 transgenic Arabidopsis expression in salt stress response genes NHX1 and LEA7 were significantly higher than that of WT. on the other hand, Si NF-YA5 transgenic Arabidopsis thaliana compared with WT for ABA the sensitivity difference is not significant, these results showed that Si mainly through NF-YA5 ABA independent way to improve the transgenic plants to high salt stress tolerance.

【作者单位】：中国农业科学院作物科学研究所/农作物基因资源与基因改良国家重大科学工程/农业部麦类生物学与遗传育种重点实验室;西北农林科技大学农学院/旱区作物逆境生物学国家重点实验室;哈尔滨师范大学生命科学与技术学院/黑龙江省分子细胞遗传与遗传育种重点实验室;
【基金】：国家转基因新品种生物培育科技重大专项(2016ZX08002-002) 中国农业科学院创新工程资助~~
【分类号】：S515;Q943.2
【正文快照】： 本研究由国家转基因新品种生物培育科技重大专项(2016ZX08002-002)和中国农业科学院创新工程资助。This work was funded by the National Majar Project for Developing New GM Crops(2014ZX08002-002)and the Innovation Project of Chi-nese Academy of Agricultural Scien

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