再生障碍性贫血中医证型与外周血端长度及TRF1，TRF2，POT1基因表达水平的相关性研究

发布时间：2018-01-12 11:12

本文关键词：再生障碍性贫血中医证型与外周血端长度及TRF1，TRF2，POT1基因表达水平的相关性研究　出处：《山东中医药大学》2016年硕士论文　论文类型：学位论文

【摘要】：目的:测定再生障碍性贫血(Aplastic Anemia,AA)患者外周血单个核细胞的端粒长度,端粒保护蛋白TRF 1、TRF 2、POT 1的mRNA表达水平;同时探讨再障中医辨证分型与外周血单个核细胞端粒长度变化、端粒保护蛋白TRF 1,TRF 2,POT 1的表达水平之间的关系,并分析端粒、TRF 1、TRF 2、POT 1之间的相关性,进一步从分子水平探讨AA的发病本质,以期为研究其发生发展及治疗寻求新的思路。方法:首先将40例AA患者按照中医辨证分型标准进行辨证分组,肾阳虚组13例、肾阴虚组13例、肾阴阳两虚组14例。并选取20例正常人外周血单个核细胞作为对照组。运用实时荧光定量聚合酶链反应(real-time quantitative polymerase chain reaction,RT-q PCR)检测患者与正常对照组外周血单个核细胞的端粒长度、端粒保护蛋TRF1,TRF2,POT1mRNA表达水平。探讨AA患者中医辨证分型与外周血单个核细胞端粒长度变化、端粒保护蛋白TRF1,TRF2,POT1 mRNA表达水平的相关性;研究AA患者外周血单个核细胞端粒长度、TRF1,TRF2,POT1之间的相关性。结果:1.再障患者TRF1,TRF2,POT1 mRNA表达水平较正常对照组升高,均有显著性差异(TRF1,TRF2,POT1,P0.05),有统计学意义;2.AA患者端粒长度变化情况较正常对照组降低,有统计学意义(P0.05);3.肾阳虚型、肾阴虚型、肾阴阳两虚型AA患者TRF1 mRNA的表达水平均较正常对照组明显升高,均有显著性差异(肾阳虚、肾阴虚型、肾阴阳两虚型,P0.01),有统计学意义;肾阳虚型、肾阴虚型AA患者TRF2 mRNA的表达水平均较正常对照组升高(肾阳虚型、肾阳虚型,P0.05),有统计学意义;肾阳虚型AA患者POT1mRNA的表达水平均较正常对照组升高(肾阳虚型P=0.039,P0.05),有统计学意义。4.肾阳虚型AA患者端粒长度变化情况均较正常对照组降低(肾阳虚型P=0.019,P0.05),有统计学意义。5.中医各证型之间,肾阴虚型TRF2mRNA表达水平较肾阴阳两虚型升高(肾阴虚型P=0.034,P0.05),有统计学意义。结论:1.TRF1,TRF2,POT1,端粒可能参与了再障的发病过程,且TRF1,TRF2,POT1高表达可能与端粒长度缩短有着密切的关系;2.端粒长度的缩短在再障的发病或疾病进展中起到重要作用,端粒长度在肾阳虚组表达最低,肾阳虚的再障患者端粒长度可能缩短更明显,端粒损耗最为严重,TRF1,TRF2,POT1很有可能与端粒缩短的过程,并可能与再障的预后有关;3.端粒长度与TRF1,TRF2表达之间呈负相关,POT1与TRF1表达之间呈正相关,提示TRF1,TRF2极有可能参与了端粒损伤缩短的过程,POT1可能通过影响TRF1而间接参与了这个过程,短端粒与再障的发病密切相关。
[Abstract]:Objective: to determine the telomere length of peripheral blood mononuclear cells (PBMC) and telomere protection protein (TRF 1) TRF2 in patients with aplastic anemia (AA). MRNA expression level of POT 1; At the same time, the relationship between TCM syndrome differentiation of aplastic anemia and the changes of telomere length in peripheral blood mononuclear cells and the expression level of telomere protection protein TRF2POT1 in peripheral blood mononuclear cells was discussed, and telomere was analyzed. The correlation between TRF 1 and TRF2POT1, further to explore the pathogenesis of AA at the molecular level. Methods: 40 patients with AA were divided into two groups according to the criteria of TCM syndrome differentiation: 13 cases in kidney-yang deficiency group and 13 cases in kidney-yin deficiency group. There were 14 cases of deficiency of kidney, yin and yang, and 20 cases of normal human peripheral blood mononuclear cells were selected as control group. Real-time fluorescence quantitative polymerase chain reaction (RPCR) was used. Real-time quantitative polymerase chain reaction. The telomere length of peripheral blood mononuclear cells and telomere protected egg TRF1 / TRF2 were measured by RT-q. To explore the relationship between TCM syndrome differentiation and telomere length of peripheral blood mononuclear cells, telomere protection protein TRF1 and TRF2 in AA patients. Correlation of POT1 mRNA expression level; To study the correlation between telomere length of peripheral blood mononuclear cells (PBMC) and TRF2POT1 in AA patients. The expression of POT1 mRNA was significantly higher than that of the control group (P 0.05). 2.The change of telomere length in AA patients was lower than that in normal control group (P 0.05). 3. The expression level of TRF1 mRNA in AA patients with deficiency of kidney yang, deficiency of kidney yin and deficiency of kidney yin and yang was significantly higher than that of normal control group, and there were significant differences (deficiency of kidney yang, deficiency of kidney yin). There was statistical significance in P0.01D of deficiency of kidney yin and yang. The expression level of TRF2 mRNA in patients with kidney yang deficiency and kidney yin deficiency was significantly higher than that in normal control group (kidney yang deficiency, kidney yang deficiency, P 0.05). The expression of POT1mRNA in AA patients with deficiency of kidney yang was higher than that in normal control group (P < 0.05). The changes of telomere length in AA patients with kidney-yang deficiency type were lower than those in normal control group (P < 0.05). The expression level of TRF2mRNA in kidney yin deficiency type was higher than that in kidney yin and yang deficiency type (P 0. 034 and P 0. 05). Conclusion: 1. TRF1, TRF2, POT1, telomere may be involved in the pathogenesis of aplastic anemia, and TRF1 may be involved in TRF2. The high expression of POT1 may be closely related to the shortening of telomere length. 2. The shortening of telomere length plays an important role in the pathogenesis or progression of aplastic anemia. Telomere length is the lowest in the group of deficiency of kidney yang, and the telomere length may be shortened more obviously in patients with aplastic anemia with deficiency of kidney yang. The most serious telomere loss was TRF1 / TRF2 / POT1, which may be related to the process of telomere shortening and the prognosis of aplastic anemia. 3. There was a negative correlation between telomere length and the expression of TRF1 and TRF2. There was a positive correlation between POT1 and TRF1 expression, indicating TRF1. TRF2 may be involved in the process of telomere injury shortening. POT1 may participate in this process indirectly by affecting TRF1. Short telomere is closely related to the pathogenesis of aplastic anemia.
【学位授予单位】：山东中医药大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R259

【相似文献】