安阳地区2013年手足口病柯萨奇病毒A6 VP1基因特征研究

发布时间：2018-01-13 16:30

本文关键词：安阳地区2013年手足口病柯萨奇病毒A6 VP1基因特征研究　出处：《病毒学报》2016年03期 　论文类型：期刊论文

【摘要】：为了解2013年安阳地区手足口病病原谱组成特点及柯萨奇病毒A6(CV-A6)VP1基因特征,采用实时荧光逆转录PCR方法对全年采集的临床手足口病例的479份粪便标本进行检测和分型,统计各型肠道病毒阳性标本数量及比例。对10份CV-A6阳性标本VP1基因进行扩增测序以获得全序列。使用BIOEDIT 7.2和MEGA 5.1软件对获得序列和NCBI数据库中检索到的参考序列进行VP1基因核苷酸、氨基酸相似性及系统发育分析。研究结果显示,检出肠道病毒阳性标本共429份,其中CV-A6阳性标本为90份,占全部阳性标本的比例为20.98%,这是安阳地区首次记录非CV-A16和EV-A71型肠道病毒列居手足口病病原谱第二位,成为手足口病主要病原之一。将成功获得的10条VP1序列与CV-A6原型株Gdula比较,发现安阳序列与Gdula VP1基因的核苷酸、氨基酸序列差异皆较大。与河南历史株HN421(2011)比较,发现有4条本地序列与HN421的核苷酸、氨基酸序列差异较大,其余6条与HN421差异较小。系统发育树显示,全部49条CV-A6序列共形成A、B、C、D四个分支。其中D分支可进一步分为D1和D2两个亚分支。安阳地区获得的10条CV-A6序列中,有6条属于D1亚分支,4条属于D2亚分支。
[Abstract]:Of hand foot and mouth disease in Anyang in 2013 to understand the spectrum and composition characteristics of Coxsackie virus A6 (CV-A6) VP1 gene characteristics, using real-time reverse transcriptase PCR method 479 specimens of the collected clinical HFMD cases were detected and classified, the statistics of enterovirus positive samples of the number and proportion of 10 CV-A6. The positive samples of VP1 gene was amplified and sequenced to obtain complete sequence. Using BIOEDIT 7.2 and MEGA 5.1 software was used to obtain VP1 gene nucleotide sequences and reference sequences retrieved from the NCBI database, the amino acid similarity and phylogenetic analysis. The results showed that the detection of intestinal virus positive samples of 429 cases, including 90 CV-A6 positive samples a total positive ratio of 20.98%, this is the first time in Anyang to record non CV-A16 and EV-A71 enterovirus ranked HFMD pathogens second, become the hand foot and mouth One of the main pathogenic disease. Will successfully obtained 10 VP1 sequences and CV-A6 prototype strains of Gdula, Gdula and VP1 showed that the nucleotide sequence of Anyang gene, amino acid sequence difference is larger. And the history of Henan strain HN421 (2011), there were 4 local sequence and HN421 nucleotide, amino acid sequence differences, the remaining 6 A and HN421 were smaller. The phylogenetic tree showed that all 49 CV-A6 sequences were formed in A, B, C, D four. The D branch branch can be further divided into D1 and D2 two sub branches. 10 CV-A6 sequences obtained in the Anyang area, 6 belonged to the D1 sub branch, 4 to the D2 sub branch.

【作者单位】：安阳市疾病预防控制中心;河南省疾病预防控制中心传染病预防控制所;
【基金】：河南省科技攻关计划项目(122102310268) 河南省医学科技重大攻关项目(201001015) 河南省卫生科技创新型人才工程中青年科技创新人才河南省安阳市科技攻关项目(2013042)
【分类号】：R725.1
【正文快照】： 手足口病最早见于20世纪50年代末,它是由多种肠道病毒引起的出疹发热性急性传染病,主要侵害对象为低龄婴幼儿。在引起手足口病的多种肠道病毒中,柯萨奇病毒A组16型(CV-A16)和肠道病毒71型(EV-A71)通常被认为是最主要的病原体,其流行范围较广,常有暴发疫情出现,危害程度较重。

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