香樟两个DREB1转录因子基因的分离及其对不同胁迫的响应分析

发布时间：2018-01-14 02:19

  本文关键词：香樟两个DREB1转录因子基因的分离及其对不同胁迫的响应分析　出处：《园艺学报》2016年04期 　论文类型：期刊论文

  更多相关文章： 香樟 CBF/DREB 基因克隆 表达分析

【摘要】：以香樟(Cinnamomum camphora)实生苗为试验材料,利用同源克隆结合RACE技术获得了两个冷诱导转录因子基因CBF/DREB1的cDNA全长序列,命名为CcCBFc和CcCBFd,Gen Bank登录号分别为KP336741和KP336742。序列分析显示这两个基因均没有内含子,cDNA全长为897和1 010 bp,开放阅读框分别为654和621 bp,编码217和206个氨基酸,预测蛋白分子量分别为24.0和22.9 kD,等电点分别为5.26和8.58。基于氨基酸序列的同源性比对和系统进化树分析表明,这两个基因均属于DREB1家族,与双子叶植物进化关系较近。实时定量PCR结果显示,CcCBFc和CcCBFd都能被低温(4℃)、干旱(20%PEG)、盐(250 mmol·L~(-1) NaCl)和ABA(100μmol·L~(-1))诱导,表明CcCBFc和CcCBFd可能在香樟应对非生物胁迫过程中发挥重要作用。
[Abstract]:The seedling of Cinnamomum camphora was used as the experimental material. The full-length cDNA sequences of two cold-induced transcription factor gene CBF/DREB1 were obtained by homologous cloning and RACE technique. They were named CcCBFc and CcCBFd. The accession numbers of Gen Bank were KP336741 and KP336742.The sequence analysis showed that the length of Gen Bank was 897 BP and 1 010 BP respectively. The open reading frames were 654 and 621 BP, encoding 217 and 206 amino acids, respectively. The predicted molecular weights of the predicted proteins were 24.0 and 22.9 KD, respectively. The isoelectric points were 5.26 and 8.58 respectively. The homologous alignment based on amino acid sequence and phylogenetic tree analysis showed that the two genes belonged to the DREB1 family. The results of real-time quantitative PCR showed that both CcCBFc and CcCBFd could be treated at 4 鈩，

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