SOX15基因对Caco2结肠癌细胞增殖、凋亡及迁移侵袭的影响

发布时间：2018-01-19 09:58

本文关键词： SOX 结肠癌转染增殖凋亡迁移侵袭　出处：《第三军医大学学报》2017年02期 　论文类型：期刊论文

【摘要】：目的检测SOX15在结肠癌细胞株中表达水平以及对细胞增殖、凋亡、迁移侵袭的影响。方法应用RT-PCR检测SOX15在Caco2、SW480、HT29、HCT116结肠癌细胞以及正常结肠组织中的表达水平。通过脂质体Lipofectamine 2000将重组质粒p EGFP-N1-SOX15及空质粒p EGFP-N1转染进Caco2细胞,应用RT-PCR及Western blot分别检测SOX15在Caco2细胞中的m RNA以及蛋白表达水平;CCK-8检测SOX15对Caco2细胞活力的影响,克隆形成实验观察细胞增殖变化,Transwell检测SOX15对细胞迁移及侵袭能力的调控,流式细胞仪分析细胞凋亡率。结果 SOX15在Caco2、SW480、HT29、HCT116结肠癌细胞中m RNA及蛋白质表达明显低于其在正常组织中的表达水平(P0.01)。p EGFP-N1-SOX15转染组细胞m RNA(1.23±0.10)和蛋白(1.13±0.08)表达显著高于p EGFP-N1对照组[(0.54±0.06)、(0.19±0.03),P0.01]及空白对照组[(0.51±0.03)、(0.14±0.02),P0.01];与空白对照组及p EGFP-N1对照组相比,p EGFP-N1-SOX15转染组可明显抑制细胞增殖及迁移侵袭能力(P0.01),并诱导细胞发生凋亡(P0.01)。结论 SOX15在结肠癌细胞中低表达,过表达SOX15可明显抑制结肠癌细胞Caco2的增殖及迁移、侵袭能力,并促进细胞凋亡。
[Abstract]:Objective to detect the expression level of SOX15 in colon cancer cell line and its effect on cell proliferation, apoptosis, migration and invasion. Methods RT-PCR was used to detect the expression of SOX15 in Caco _ 2 / SW480 cells. HT29. Expression level of HCT116 colon cancer cells and normal colon tissue. Recombinant plasmid p. EGFP-N1-SOX15 and empty plasmid p EGFP-N1 were transfected into Caco2 cells. The expression of m RNA and protein in Caco2 cells was detected by RT-PCR and Western blot, respectively. CCK-8 was used to detect the effect of SOX15 on the viability of Caco2 cells. Clone formation assay was used to observe the proliferation of Caco2 cells. Transwell was used to detect the regulation of SOX15 on cell migration and invasion, and the apoptosis rate was analyzed by flow cytometry. Results SOX15 was detected in Caco2, SW480 and HT29. The expression of m RNA and protein in HCT116 colon cancer cells was significantly lower than that in normal tissues. The expression of m RNA(1.23 卤0. 10) and protein 1. 13 卤0. 08) in p EGFP-N1-SOX15 transfected group was significantly higher than that in p EGFP-N1 control group. [0. 54 卤0. 06 and 0. 19 卤0. 03 P0.01) and blank control group. [(0. 51 卤0. 03) and 0. 14 卤0. 02 (P0.01); Compared with the blank control group and the p EGFP-N1 control group, the transfection group of p EGFP-N1-SOX15 could significantly inhibit the cell proliferation and migration and invasion ability (P0.01). Conclusion the expression of SOX15 in colon cancer cells is low, and overexpression of SOX15 can significantly inhibit the proliferation and migration of Caco2 in colon cancer cells. Invasive ability and promote apoptosis.
【作者单位】：重庆医科大学附属第一医院消化内科;
【分类号】：R735.3
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