MicroRNA及其靶基因在糖尿病肾病小鼠肾脏的表达

发布时间：2018-01-19 21:52

本文关键词： 糖尿病肾病 microRNA 实时荧光定量PCR 靶基因　出处：《科技导报》2017年04期 　论文类型：期刊论文

【摘要】：验证与糖尿病肾病小鼠肾脏相关的microRNAs的表达并运用实时荧光定量PCR分析靶基因与糖尿病肾病的关系。以db/db小鼠为模型组(DN组),db/m小鼠为正常组(NC组),定期测量小鼠的体重、血糖、甘油三酯、总胆固醇及24 h尿蛋白排泄率。留取DN小鼠与NC小鼠肾脏组织,检测肾脏组织形态学染色及实时荧光定量PCR(qRT-PCR)。qRT-PCR验证差异表达的microRNAs及其靶基因的m RNA表达水平。血糖、24 h尿蛋白排泄率结果表明糖尿病肾病动物模型构建成功。与NC小鼠相比,DN小鼠肾脏miR-196a、miR-21、miR-200b表达明显升高,且差异有统计学意义(P0.05)。miR-196a、miR-200b、miR-21的表达水平与血糖、甘油三酯、总胆固醇、24 h尿蛋白排泄率存在正相关关系(P0.05)。利用miRNAs数据库预测miR-196a的靶基因有ANX1、HOXB7、PTEN、FOXO1、HOXB8、HOXA5等。与NC组比较,DN组ANX1、FOXO1的m RNA表达水平降低,且差异有统计学意义(P0.05)。同时ANX1、FOXO1与24 h尿蛋白排泄率存在正相关(P0.05)。MiR-196a可能通过调节ANX1、FOXO1的表达水平来参与糖尿病肾病的发生发展。
[Abstract]:To verify the expression of microRNAs related to kidney in diabetic nephropathy mice and to analyze the relationship between target gene and diabetic nephropathy by real-time fluorescence quantitative PCR. DN group. The body weight, blood glucose, triglyceride, total cholesterol and 24 h urinary protein excretion rate of db/m mice were measured regularly. Kidney tissues of DN mice and NC mice were collected. Detection of renal histomorphology and real-time fluorescence quantitative PCRQRT-PCR). The expression level of m RNA of differentially expressed microRNAs and its target gene was confirmed by qRT-PCR. The results of 24 h urinary protein excretion rate showed that the animal model of diabetic nephropathy was successfully constructed, and the renal miR-196a1 miR-21 of DN mice was compared with that of NC mice. The expression of miR-200b was significantly increased, and the difference was statistically significant (P 0.05). The expression level of miR-200b miR-21 and blood glucose, triglyceride were significantly higher than those in control group. There was a positive correlation between urinary protein excretion rate of 24 h and total cholesterol excretion rate (P0.05A). The target gene of miR-196a was ANX1, HOXB7, which was predicted by miRNAs database. Compared with NC group, the expression level of m RNA of ANX1 / FOXO1 in DN group was lower than that in NC group. The difference was statistically significant (P 0.05) and ANX1. There was a positive correlation between FOXO1 and 24 h urinary protein excretion rate (P 0.05). MiR-196a may regulate ANX1. The expression of FOXO1 is involved in the development of diabetic nephropathy.
【作者单位】：新疆医科大学基础医学院;
【基金】：国家自然科学基金项目(81460162) 高等学校科研计划项目(XJEDU2014I021)
【分类号】：R587.2;R692.9
【正文快照】： 糖尿病(diabetes mellitus,DM)是一种以高血糖为特征的慢性代谢性疾病。糖尿病肾病(diabetic nephropathy,DN)是糖尿病患者常见的微血管并发症之一,也是引起终末期肾脏功能衰竭(end-stage renal disease,ESRD)的重要诱因[1-3]。DN的发病与遗传、代谢、肾脏血流动力学、高血压

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