冠突散囊菌nsdD基因超表达菌株的构建及表型分析

发布时间：2018-01-20 03:40

本文关键词： 冠突散囊菌表达质粒构建 nsd D基因超表达农杆菌介导　出处：《基因组学与应用生物学》2017年03期 　论文类型：期刊论文

【摘要】：为了研究冠突散囊菌nsdD基因的功能,本研究将冠突散囊菌nsdD的cDNA置于强启动子三磷酸甘油醛脱氢酶启动子PgpdA下游及色氨酸合成酶C基因终止子TtrpC上游构建nsdD基因超表达载体PURT5750,采用根癌农杆菌介导方法将表达质粒PURT5750转化到冠突散囊菌进行功能验证。结果显示向基因组随机插入外源质粒DNA,随机挑选11个nsd D基因超表达转化子,对nsdD基因超表达转化子菌株培养观察并与野生型菌株相比发现,在5%和17%NaCl培养基中菌落差异不显著,而且仍能产生成熟的有性孢子。本实验为冠突散囊菌有性发育调控机制的研究提供了技术基础。
[Abstract]:In order to study the function of nsdD gene in Candida coronalis. In this study, the cDNA of nsdD was placed downstream of the strong promoter glyceraldehyde triphosphate dehydrogenase promoter PgpdA and the terminal TtrpC of tryptophan synthase C gene to construct nsdD gene. PURT5750. Agrobacterium tumefaciens mediated transformation of the expression plasmid PURT5750 to sporocystis coronatum for functional verification. The results showed that the genome was randomly inserted into the exogenous plasmid DNA. Eleven nsdD gene overexpression transformants were randomly selected and compared with wild-type strains. There was no significant difference in colony between 5% and 17 NaCl medium, and mature sexual spores could still be produced. This study provided a technical basis for the study of the regulation mechanism of sexual development of Candida coronatum.
【作者单位】：湖北医药学院基础医学院微生物学教研室;贵州省科学技术厅;十堰市太和医院普外科;贵州省农业生物技术重点实验室;贵州省生物技术研究所;贵州省农业科学院;
【基金】：十堰市科技局项目(ZD2012019) 湖北医药学院校基金(2014QDJZR15)共同资助
【分类号】：Q78;Q93
【正文快照】： *通讯作者,gzliuzuoyi@163.comConstruction and Phenotypic Analysis of Over-Expression Strain of nsd DGene in Eurotium cristatumYu Chunfang 1 Xiong Qing 2 Li Wenfang 3 Tan Yumei 4,5 Liu Yongxiang 4,5 Liu Zuoyi 4,6*Ou Qing 1*1 Department of Microbiology,Facu

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