AANAT基因编码区多态性与家兔生长性状的关联分析

发布时间：2018-01-20 13:42

本文关键词： 家兔 AANAT 单核苷酸基因多态性生长性状　出处：《中国畜牧杂志》2017年10期 　论文类型：期刊论文

【摘要】：为研究五羟色胺-N-乙酰基转移酶(AANAT)基因编码区(CDS区)多态性和家兔生长性状的关联性,以天府黑兔、伊拉兔和新西兰兔作为实验材料,对AANAT基因的外显子进行PCR扩增,直接测序检测家兔AANAT基因外显子的单核苷酸多态性。结果表明:在3个兔品种的CDS区上均发现有5个完全连锁突变位点,分别是C.132TC、C.153TC、C.159 AT、C.234 AG、C.291 AG,形成了野生型TTTTAAAAAA、杂合型TCTCATAGAG、突变型CCCCTTGGGG 3种基因型,突变型新西兰兔在56、70、84日龄的体重显著高于野生型和杂合型(P0.05),而突变型伊拉兔和天府黑兔在70、84日龄的体重显著高于杂合型和野生型(P0.05)。综上可知,AANAT基因可作为肉兔生长性状的候选基因。
[Abstract]:In order to study the association between polymorphism of serotonin N-acetyltransferase (AANATE) gene coding region and growth traits of rabbits, Tianfu black rabbit, Ira rabbit and New Zealand rabbit were used as experimental materials. The exon of AANAT gene was amplified by PCR. The single nucleotide polymorphisms of the exon of AANAT gene in rabbits were detected by direct sequencing. The results showed that there were five completely linked mutation sites in the CDS region of the three rabbits, namely C.132TC. The wild type TTTTAAAA, heterozygote TCTCATAGAG was formed. The body weight of mutant New Zealand rabbits was significantly higher than that of wild type and heterozygote (P0.05). However, the body weight of mutant Ira rabbits and Tianfu black rabbits was significantly higher than that of heterozygote and wild type P0.05N at 70 ~ 84 days old, which suggested that AANAT gene could be used as a candidate gene for growth traits of meat rabbits.
【作者单位】：四川农业大学动物遗传育种研究所;
【基金】：四川省科技支撑计划项目(2016NYZ0046、2016NYZ0050)
【分类号】：Q78;S829.1
【正文快照】： 我国是养兔大国,也是兔肉出口大国。家兔是一种非常重要的经济动物,它既可以食用,也可以作为工业原料、观赏动物、医用模型等。五羟色胺-N-乙酰基转移酶(AANAT)是褪黑激素(Melatonin,MT)合成过程的限速酶[1]。MT在生物体内具有调控动物的生长、应激、炎症、昼夜节律、视网膜信

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