南丹瑶鸡PGC-PGC-1α基因SNP效应的初步分析

发布时间：2018-01-20 14:31

本文关键词： 广西南丹瑶鸡脂肪沉积 PGC-α PPARγ　出处：《中国家禽》2017年04期 　论文类型：期刊论文

【摘要】：为探讨过氧化物酶体增殖物激活受体γ辅激活子(PGC-1α)基因646位点突变对PGC-1α和过氧化物酶体增生物激活受体(PPARγ)基因表达的影响,利用实时荧光定量PCR技术检测了PGC-1α和PPARγ基因在南丹瑶鸡PGC-1α基因646位点AA、AG和GG三种基因型的腹脂、皮脂和肝脏中的表达。结果显示,在肝脏中,PGC-1α和PPARγ在AA型中的表达量都显著高于GG型和GA型(P0.05);在皮脂中,PGC-1α基因在AA型的表达量显著高于AG型和GG型(P0.05),而PPARγ在AG型和GG型中的表达量则显著高于AA型(P0.05);在腹脂中,PGC-1α和PPARγ基因在AG型和GG型中的表达量都显著高于AA型(P0.05)。说明PGC-1α基因646位点的突变影响了南丹瑶鸡PGC-1α和PPARγ基因的表达,结果为揭示广西南丹瑶鸡皮脂薄的分子机理和培育低脂优质的肉鸡品种奠定了基础。
[Abstract]:To investigate the effects of peroxisome proliferator-activated receptor 纬 co-activator (PGC-1 伪) gene 646 mutation on PGC-1 伪 and peroxisome proliferator activated receptor (PPAR- 纬). The effect of gene expression. The abdominal fat of PGC-1 伪 and PPAR 纬 gene in PGC-1 伪 gene 646 locus of Nandan Yao chicken was detected by real-time fluorescence quantitative PCR technique. The results showed that the expressions of PGC-1 伪 and PPAR 纬 in liver were significantly higher than those in GG and GA P0.05a. In sebum, the expression of PGC-1 伪 gene in AA was significantly higher than that in AG and GG (P0.05). The expression of PPAR 纬 in AG and GG type was significantly higher than that in AA type P 0.05; In abdominal fat. The expression levels of PGC-1 伪 and PPAR 纬 genes in AG and GG were significantly higher than those in AA type (P0.05). The results showed that the mutation of PGC-1 伪 gene at 646 site affected the expression of PGC-1 伪 and PPAR 纬 genes in Nandan Yao chicken. The results laid a foundation for revealing the molecular mechanism of sebum thinning in Guangxi Nandan Yao chicken and for breeding low fat and high quality broiler breeds.
【作者单位】：广西大学动物科学技术学院;广西壮族自治区畜牧研究所;广西贵港市港丰农牧有限公司;
【基金】：广西自然科学基金项目(2014GXNSFAA118133) 广西区水产畜牧局科技项目([桂渔牧科(201633008)]) 广西水产畜牧科技推广应用项目([桂渔牧科(201633025)])
【分类号】：S831.2
【正文快照】： 肉鸡经过几十年的选育,生长速度和生产性能得到很大提高的同时,也带来了腹脂和皮脂沉积增加,肉质下降等负面影响[1]。随着人们生活水平的提高,低脂优质的肉鸡成为人们消费的主流方向。控制脂肪在鸡体内的过度沉积,改善禽肉品质成为目前育种工作的主要奋斗目标之一。南丹瑶鸡产

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