Zeste基因增强子人类同源物2抑制剂GSK343调节巨噬细胞分化的作用

发布时间：2018-01-20 20:28

本文关键词： 巨噬细胞牙周炎表观遗传学微环境　出处：《华西口腔医学杂志》2017年03期 　论文类型：期刊论文

【摘要】：目的研究Zeste基因增强子人类同源物2(EZH2)抑制剂GSK343调节巨噬细胞亚群的分化,探讨EZH2在牙周炎中潜在的治疗作用。方法将巨噬细胞RAW264.7分为4组:空白组(A组)、对照组(B组)、内毒素(LPS)刺激组(C组)、LPS+GSK343组(D组)。细胞经培养及相应处理后,利用免疫印迹和酶联免疫吸附试验检测其表型生物学标志变化,包括肿瘤坏死因子-α(TNF-α)、诱导型一氧化氮合酶(i NOS)、白细胞介素-10(IL-10)和精氨酸酶-1(Arg-1)。利用大肠杆菌吞噬试验检测巨噬细胞RAW264.7在不同条件下对大肠杆菌的吞噬作用。结果 LPS可以诱导RAW264.7产生M1表型生物标志(TNF-α和i NOS表达增加),在加入EZH2抑制GSK343后,IL-10和Arg-1表达升高,提示EZH2抑制剂GSK343可以诱导RAW264.7细胞由M1型向M2型转化;RAW264.7细胞具有吞噬大肠杆菌的作用,加入LPS的条件下吞噬大肠杆菌作用加强,而EZH2抑制剂GSK343可以调节RAW264.7细胞对大肠杆菌的吞噬作用。结论 EZH2抑制剂GSK343可以调节巨噬细胞的分化,在牙周炎的治疗中可能具有潜在作用。
[Abstract]:Objective to study the effect of Zeste gene enhancer GSK343 on the differentiation of macrophage subsets. To explore the potential therapeutic effect of EZH2 in periodontitis. Methods macrophage RAW264.7 was divided into 4 groups: blank group (A group) and control group (group B). Lipopolysaccharide (LPS) stimulated group C was treated with lipopolysaccharide (GSK343) and treated with lipopolysaccharide (LPS). Immunoblotting and enzyme linked immunosorbent assay (Elisa) were used to detect the changes of phenotypic markers, including tumor necrosis factor- 伪 (TNF- 伪) and inducible nitric oxide synthase (iNOS). Interleukin-10 (IL-10) and argininase 1 (Arg-1). The phagocytic effect of macrophage RAW264.7 on Escherichia coli under different conditions was detected by Escherichia coli phagocytosis test. LPS can induce RAW264.7 to produce M1 phenotypic biomarkers (. The expression of TNF- 伪 and I NOS was increased. The expression of IL-10 and Arg-1 increased after GSK343 was inhibited by adding EZH2. It is suggested that EZH2 inhibitor GSK343 can induce the transformation of RAW264.7 cells from M1 to M2. RAW264.7 cells have phagocytic effect of Escherichia coli, the addition of LPS enhanced the phagocytosis of Escherichia coli. EZH2 inhibitor GSK343 can regulate the phagocytosis of RAW264.7 cells to Escherichia coli. Conclusion EZH2 inhibitor GSK343 can regulate the differentiation of macrophages. It may play a potential role in the treatment of periodontitis.
【作者单位】：西南医科大学口颌面修复重建和再生实验室;西南医科大学附属口腔医院口腔内科;西南医科大学附属口腔医院修复科;
【基金】：泸州市科技局基金[2016-S-66(1/3)]~~
【分类号】：R781.42
【正文快照】： 2.西南医科大学附属口腔医院口腔内科;3.修复科,泸州646000巨噬细胞是免疫系统的重要成分之一,在机体的先天性免疫反应和获得性免疫反应中起着关键作用,对机体内稳态环境平衡具有重要意义。它能够侦测入侵病原体和异质性抗原,启动先天性免疫反应,且其抗原加工递呈作用对获得性

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