小麦品系XN6426抽穗期相关基因的检测与定位

发布时间：2018-01-21 11:31

  本文关键词： 小麦 抽穗期 基因定位 SSR BSA　出处：《麦类作物学报》2016年11期 　论文类型：期刊论文

【摘要】：为定位小麦品系XN6426抽穗期相关基因,在可控温室(温度16~25℃,日光照≥16h)和田间分别种植XN6426×京411F2代群体、早抽穗亲本XN6426和晚抽穗亲本京411,分析F2群体抽穗期表型,得出该表型由两对基因控制。构建温室条件下F2群体的极端早抽穗和极端晚抽穗期DNA池(BSA法),采用小麦90KSNP芯片分析得出早晚抽穗期池间差异SNP位点在不同染色体上的分布频率和相应密集区域;在5A染色体上筛选出双亲和早晚抽穗期池间有多态性且分布在差异SNP位点密集区域附近的SSR标记Xbarc151和Xwmc327,这两对标记检测群体的基因型与其抽穗期表型之间极显著负相关。检测Xbarc151、Xwmc327以及亲本间有多态性的标记Xgwm186和Xwmc96在F2群体内的基因型,并结合群体相应抽穗期表型,利用复合区间作图法,在5A染色体标记Xbarc151和Xwmc327之间检测到了1个抽穗期相关QTL位点qHD-5A-1,距离两标记的遗传距离分别为1.00cM和11.49cM,LOD值为3.68,贡献率为8.07%,结合前人结果初步确定该位点与Vrn-A1位点不同,可能为已知抽穗期相关基因的未知等位变异或新基因位点。
[Abstract]:For the location of wheat lines XN6426 heading related gene, in a controlled greenhouse (temperature 16~25, sunlight is no less than 16h) and in the field were planted XN6426 * Beijing 411F2 generation group, early heading parent XN6426 and late heading parent Beijing 411, F2 group analysis of phenotype, the phenotype was controlled by two pairs of genes to construct F2. The group under greenhouse conditions of extreme early heading and extreme late heading date in the DNA pool (BSA), using wheat 90KSNP chip analysis of distribution frequency differences between SNP sooner or later heading cell sites in different chromosomes and corresponding dense regions on chromosome 5A; selected parents and sooner or later heading stage between the polymorphism and the distribution of pool the difference in SNP Xbarc151 and Xwmc327 SSR marker loci concentrated near the area, between the genotypes on the two groups with markers of phenotype significantly negatively correlated. Detection of Xbarc151, Xwmc327 and polymorphisms between the parent The markers Xgwm186 and Xwmc96 in the F2 group within the group and combined with the corresponding genotype, phenotype, using composite interval mapping method between 5A chromosome markers Xbarc151 and Xwmc327 detected 1 QTL heading loci qHD-5A-1, the genetic distance of two marker distance were 1.00cM and 11.49cM, LOD value was 3.68. The contribution rate of 8.07%, with the previous results to determine the site and Vrn-A1 site, may be heading the unknown genes related to known alleles or loci.

【作者单位】： 西北农林科技大学农学院;宁夏农林科学院农作物研究所;
【基金】：国家重点基础研究发展计划(973计划)项目(2104CB138102) 陕西省重点科技创新团队计划项目(2014KCT-25) 宁夏农业育种专项(2013NYYZ02) 宁夏农林科学院科技创新先导资金对外科技合作专项-小麦品质与抗逆性相关的多重PCR分子标记体系引进与利用;宁夏农林科学院科技创新先导资金基础研究项目(NKYJ-14-29) 西北农林科技大学唐仲英育种基金项目
【分类号】：S512.1
【正文快照】： of the polymorphism SNP loci,and the genotype detected by the two SSR markers was significantlynegative correlated with the phenotype of heading.The genotype detected by Xbarc151,Xwmc327andthe two markers Xgwm186,Xwmc96which were polymorphism between the，

本文编号：1451358